A sensitive method has been developed for simultaneous determination of ginsenoside Rh₁ (G-Rh₁), ginsenoside Rb₁ (G-Rb₁), ginsenoside Rc (G-Rc), and ginsenoside Rd (G-Rd) in rat plasma of normal and depression model group after oral administration of their solutions by using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry (UHPLC-QQQ-MS). The biological samples were prepared by protein precipitation. Ginsenoside Rg₃ (G-Rg₃) was used as an internal standard (IS). MS analysis was performed under the multiple reaction monitoring (MRM) with electron spray ionization (ESI) operated in the negative mode. The method showed good linearity over a wide concentration range (R² > 0.999) and obtained lower limits of quantification (LLOQ) of 5 ng/mL. The whole analysis procedure could be completed in as short as 16.5 min. The intraday precisions, interday precisions, and stabilities were less than 10%. The extraction recoveries from rat plasma were exceeded 86.0%. The results indicated that there were significant differences between the two groups on pharmacokinetics parameters; the absorptions of four analytes in the depression group were higher than those in the normal group because the liver metabolism and internal environment of the model rats had been affected.

中文翻译：

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大鼠血浆中四种人参皂苷的同时定量及其在使用 UHPLC-MS/MS 的正常和抑郁大鼠的药代动力学比较研究中的应用

已开发出一种灵敏的方法来同时测定大鼠血浆中的人参皂苷 Rh ₁ (G-Rh ₁ )、人参皂苷 Rb ₁ (G-Rb ₁ )、人参皂苷 Rc (G-Rc) 和人参皂苷 Rd (G-Rd)使用超高效液相色谱-串联质谱（UHPLC-QQQ-MS）口服其溶液后的正常和抑郁模型组。生物样品通过蛋白质沉淀制备。人参皂苷 Rg ₃ (G-Rg ₃) 用作内标 (IS)。MS 分析在多反应监测 (MRM) 下进行，电子喷雾电离 (ESI) 在负模式下运行。该方法在较宽的浓度范围内显示出良好的线性 ( R ² > 0.999) 并获得了 5 ng/mL 的定量下限 (LLOQ)。整个分析过程可在短短 16.5 分钟内完成。日内精密度、日间精密度和稳定性均小于10%。从大鼠血浆中提取回收率超过 86.0%。结果表明，两组药代动力学参数存在显着差异；由于模型大鼠肝脏代谢和内环境受到影响，抑郁组四种分析物的吸收均高于正常组。