Abstract

In recent years, magnesium calcium phosphate materials have been considered as an alternative to calcium phosphate-based materials in reconstructive surgery. In this work, bone cements in the calcium phosphates–magnesium phosphates system were synthesized and studied. The interaction of this system with a cement liquid gives struvite MgNH₄PO₄·6H₂O as the main phase. The obtained materials have a compressive strength of up to 54 ± 5 MPa, a setting time of 6–7 min, and a neutral pH value. The effect of the introduction of vancomycin to the cement materials was studied. Investigation of the kinetics of vancomycin release showed that up to 98% of the antibiotic is released within 21 days. The materials exhibited pronounced antibacterial activity against the Staphylococcus aureus and Escherichia coli strains. After the introduction of vancomycin, the zone of inhibition of bacterial growth more than doubled compared to the reference samples. In vivo tests were conducted, the structural factors were calculated based on the results of micro-CT. According to macro and micro signs, the cement materials are fully biocompatible; by the sixth week, the formation of new bone tissue is observed.

中文翻译：

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基于鸟粪石的骨水泥：万古霉素负载的影响和体内生物相容性和骨传导潜力的评估

摘要

近年来，磷酸镁钙材料被认为是重建手术中磷酸钙基材料的替代品。在这项工作中，合成并研究了磷酸钙-磷酸镁体系中的骨水泥。该体系与水泥液体的相互作用使鸟粪石 MgNH ₄ PO ₄ ·6H ₂ O 作为主相。所得材料的抗压强度高达 54±5 MPa，凝固时间为 6-7 分钟，pH 值为中性。研究了在水泥材料中引入万古霉素的效果。对万古霉素释放动力学的研究表明，高达 98% 的抗生素在 21 天内释放。材料表现出明显的抗菌活性金黄色葡萄球菌和大肠杆菌菌株。引入万古霉素后，与参考样品相比，细菌生长的抑制区增加了一倍以上。进行了体内试验，根据显微CT的结果计算了结构因素。根据宏观和微观迹象，水泥材料具有完全的生物相容性；到第六周，观察到新骨组织的形成。