Plant-based medicines have been a part of primary healthcare since time immemorial. Natural products have received increasing attention to treat several regenerative diseases due to their availability and fewer side effects than conventional drugs. Towards this, Neem, commonly known as ‘Divine Life-giving tree’, has been investigated for its efficiency against MCF7, breast cancer cell lines. Ethanolic neem extracts were analysed for qualitative phytochemical screening tests, spectral analysis, antioxidant activity, and total phenol contents. In addition, to enhance the recovery of secondary metabolites from the plant extracts, electroporation (EP) technique is used. The effect of EP was studied by comparing with untreated extracts, which exhibits antioxidant activity (∼96% increase) and total phenol contents (∼12.51% increase) than untreated extracts. This indicates that electroporated extract (EE) exhibited higher antioxidant and phenolic activity than other extracts. Furthermore, different concentrations of untreated and electroporated neem extracts were used to study the anti-cancer activity. To enhance the uptake of extracts against cell membranes, electrical pulses of EP1 = 1200 V/cm, 100 s, 8 pulses and, EP2 = 500 V/cm, 20 ms, 8 pulses were used. The results indicate cell viabilities of 35% with untreated extract only, 5.6% with EE, 3.17%, and 2.49% with EP1 + EE extract and EP2 + EE extract, respectively, indicating the potential of the synergy of electrical pulses + EE. The viabilities were lowered by 5–6 times after 72 h. This treatment is more appropriate to achieve maximum cell death, thereby reducing the side effects of conventional drugs in treating breast cancer.

自古以来，植物性药物一直是初级保健的一部分。天然产品由于其可用性和比传统药物更少的副作用而受到越来越多的关注，用于治疗多种再生性疾病。为此，人们对印楝（通常被称为“神圣的生命树”）针对 MCF7 乳腺癌细胞系的功效进行了研究。对乙醇印楝提取物进行定性植物化学筛选测试、光谱分析、抗氧化活性和总酚含量进行分析。此外，为了提高植物提取物中次生代谢物的回收率，还使用了电穿孔（EP）技术。通过与未经处理的提取物进行比较来研究 EP 的效果，未处理的提取物表现出抗氧化活性（增加约 96%）和总酚含量（约 12. 比未经处理的提取物增加了 51%）。这表明电穿孔提取物（EE）比其他提取物表现出更高的抗氧化和酚活性。此外，使用不同浓度的未经处理和电穿孔的印楝提取物来研究其抗癌活性。为了增强细胞膜对提取物的吸收，使用电脉冲 EP1 = 1200 V/cm，100 秒，8 个脉冲，EP2 = 500 V/cm，20 ms，8 个脉冲。结果表明，未经处理的提取物的细胞活力分别为 35%、EE 的细胞活力为 5.6%、EP1 + EE 提取物和 EP2 + EE 提取物的细胞活力分别为 3.17% 和 2.49%，表明电脉冲 + EE 协同作用的潜力。72小时后活力下降5-6倍。这种治疗更适合实现最大限度的细胞死亡，