Rapid diagnosis plays a vital role in daily life and is effective in reducing treatment costs and increasing curability, especially in remote areas with limited availability of resources. Among the various common methods of rapid diagnosis, centrifugal microfluidics has many unique advantages, such as less sample consumption, more precise valve control for sequential loading of samples, and accurately separated module design in a microfluidic network to minimize cross-contamination. Therefore, in recent years, centrifugal microfluidics has been extensively researched, and it has been found to play important roles in biology, chemistry, and medicine. Here, we review the latest developments in centrifugal microfluidic platforms in immunoassays, biochemical analyses, and molecular diagnosis, in recent years. In immunoassays, we focus on the application of enzyme-linked immunosorbent assay (ELISA); in biochemical analysis, we introduce the application of plasma and blood cell separation; and in molecular diagnosis, we highlight the application of nucleic acid amplification tests. Additionally, we discuss the characteristics of the methods under each platform as well as the enhancement of the corresponding performance parameters, such as the limit of detection, separation efficiency, etc. Finally, we discuss the limitations associated with the existing applications and potential breakthroughs that can be achieved in this field in the future.

中文翻译：

---

离心微流控技术在免疫分析、生化分析和分子诊断中的应用

快速诊断在日常生活中起着至关重要的作用，可有效降低治疗成本和提高治愈率，尤其是在资源有限的偏远地区。在各种常见的快速诊断方法中，离心微流控具有许多独特的优势，例如样品消耗量少，样品顺序加载的阀门控制更精确，以及微流控网络中精确分离的模块设计以最大程度地减少交叉污染。因此，近年来离心微流体得到了广泛的研究，并发现它在生物学、化学和医学中发挥着重要作用。在这里，我们回顾了近年来离心微流控平台在免疫测定、生化分析和分子诊断方面的最新发展。在免疫测定中，我们专注于酶联免疫吸附试验（ELISA）的应用；在生化分析中，我们介绍了血浆和血细胞分离的应用；在分子诊断方面，我们强调核酸扩增检测的应用。此外，我们讨论了每个平台下方法的特点以及相应性能参数的增强，例如检测限、分离效率、等。最后，我们讨论了与现有应用程序相关的局限性以及未来在该领域可以实现的潜在突破。