In this study, halloysite nanotubes (HNTs) were subjected to surface functionalization using sodium alginate and incorporated into poly(caprolactone) (PCL) to fabricate nanocomposites for potential wound healing applications. The nanocomposite films were fabricated through the solution casting technique and characterized using various instrumental methods. The films exhibited enhanced thermal and mechanical properties. FE-SEM and AFM analyses confirmed the uniform dispersion of the HNTs and increased roughness of the films, respectively. The swelling properties, in-vitro enzymatic degradation, and anti-inflammatory activity of the films were also analyzed. The MTT assay performed using NIH3T3 cell lines revealed enhanced cell proliferation (126 ± 1.38) of 5 wt% film. Besides, the cell adhesion tests of the films revealed their cytocompatibility. The scratch assay tests conducted for observing the effectiveness of the films for wound closure showed that the 5 wt% film offered a higher rate of fibroblast cell migration (32.24 ± 0.49) than the pristine PCL film. The HRBCMS assay demonstrated the hemocompatibility of these films. The biological test results indicated the delayed enzymatic degradability and haemocompatiblity of nanocomposites with enhanced cell adhesion, cell proliferation, and cell migration capabilities with respect to fibroblast cells. In summary, the synthesized nanocomposite films can be effectively used in wound healing applications after further clinical trials.

在这项研究中，埃洛石纳米管 (HNT) 使用海藻酸钠进行表面功能化，并结合到聚（己内酯）（PCL）中以制造用于潜在伤口愈合应用的纳米复合材料。纳米复合薄膜是通过溶液浇铸技术制造的，并使用各种仪器方法进行表征。薄膜表现出增强的热性能和机械性能。FE-SEM 和 AFM 分析分别证实了 HNT 的均匀分散和薄膜粗糙度的增加。溶胀特性，体外还分析了膜的酶促降解和抗炎活性。使用 NIH3T3 细胞系进行的 MTT 测定显示 5 wt% 薄膜的细胞增殖增强 (126 ± 1.38)。此外，薄膜的细胞粘附测试揭示了它们的细胞相容性。为观察伤口闭合薄膜的有效性而进行的划痕试验表明，5 wt% 的薄膜提供了比原始 PCL 薄膜更高的成纤维细胞迁移率 (32.24 ± 0.49)。HRBCMS 测定证明了这些薄膜的血液相容性。生物学测试结果表明，纳米复合材料具有延迟的酶促降解性和血液相容性，具有增强的细胞粘附、细胞增殖和细胞迁移能力相对于成纤维细胞。总之，