Pinellia ternata (Thunb.) Breit. is a commonly used herb in traditional Chinese medicine, and the main raw material of various Chinese patent medicines. Continuous cropping obstacle (CCO) is the main factor leading to the decline of crop yields and quality. Metagenomics sequencing technology was used to analyze the microbial community and functional genes of continuous cropping (CC) and control (CK) soils of P. ternata. In addition, differences in physicochemical properties, enzyme activities, microbial community composition and the abundance of functional genes in CC and CK were evaluated, as well as the relationship between these factors and CCO. Results indicated that CC of P. ternata led to the decline of rhizosphere soil pH, nutrient imbalance and enzyme activity reduction. Metagenomic analysis indicted that CC also changed the composition of the microbial community, causing an increase in the relative abundance of pathogenic microorganisms such as Fusarium, Klebsiella oxytoca and Pectobacterium carotovorum in the P. ternata rhizosphere. The relative abundance of potentially beneficial Burkholderia and Bradyrhizobium was recorded to decrease. Results also showed that there were considerable differences in CC and CK about the abundances of functional genes related to soil enzymes and the degradation of P. ternata allelochemicals, as well as the microbial groups which they belong. These results clarified the effects of CC on the microbial community structure and functional genes of soil. In addition, Burkholderia and Bradyrhizobium might play important roles in enhancing soil fertility and reducing the toxicity of phenolic acids in rhizosphere soil. CC of P. ternata changed the physicochemical properties, microbial community and functional genes of rhizosphere soil. Burkholderia and Bradyrhizobium for enhancing soil fertility and reducing the toxicity of phenolic acids might be potentially beneficial. These results provide theoretical guidance for bioremediation of CCO soil of P. ternata and other staple crops.

中文翻译：

---

连作半夏 (Thunb.) Breit 的效果。土壤理化性质、酶活性、微生物群落和功能基因

半夏 (Thunb.) Breit。是一种常用的中药材，是各种中成药的主要原料。连作障碍（CCO）是导致作物产量和品质下降的主要因素。采用宏基因组学测序技术，分析了黑松连作（CC）和对照（CK）土壤的微生物群落和功能基因。此外，还评估了 CC 和 CK 在理化性质、酶活性、微生物群落组成和功能基因丰度方面的差异，以及这些因素与 CCO 之间的关系。结果表明，P. ternata 的 CC 导致根际土壤 pH 值下降、养分失衡和酶活性降低。宏基因组分析表明，CC 还改变了微生物群落的组成，导致 P. ternata 根际中病原微生物的相对丰度增加，如镰刀菌、催产克雷伯菌和食果肉杆菌。潜在有益的伯克霍尔德菌和慢生根瘤菌的相对丰度被记录到减少。结果还表明，CC和CK在与土壤酶相关的功能基因的丰度和P. ternata化感化学物质的降解以及它们所属的微生物群方面存在相当大的差异。这些结果阐明了CC对土壤微生物群落结构和功能基因的影响。此外，Burkholderia 和 Bradyrhizobium 可能在提高土壤肥力和降低根际土壤酚酸毒性方面发挥重要作用。P. ternata 的 CC 改变了根际土壤的理化性质、微生物群落和功能基因。Burkholderia 和 Bradyrhizobium 用于提高土壤肥力和降低酚酸的毒性可能是有益的。这些结果为紫杉和其他主要作物的CCO土壤的生物修复提供了理论指导。