Non-human primates (NHP) are essential in modern biomedical research; New World monkeys (NWM) are mainly used as an experimental model regarding human malaria as they provide useful information about the parasite’s biology and an induced immune response. It is known that a vaccine candidate’s efficacy is mediated by a protection-inducing antibody response (IgG).

Not enough information is available concerning IgG subclasses’ molecular characteristics regarding NHP from parvorder Platyrrhini. Understanding the nature of the humoral immune response and characterising the IgG subclasses’ profile will provide valuable information about the immunomodulator mechanisms of vaccines evaluated using an NHP animal model.

This article has characterised IgG subclasses in NWM (i.e. genera Aotus, Cebus, Ateles and Alouatta) based on the amplification, cloning and sequencing of the immunoglobulin heavy constant gamma (IGHG) gene’s CH1 to CH3 regions. The resulting sequences enabled elucidating IGHG gene organisation; two IgG variants were found in the Aotus and Ateles monkey group and three IgG variants in the Cebus and Alouatta group. The sequences were highly conserved in Platyrrhini and had a similar structure to that reported for monkeys from parvorder Catarrhini. Such information will help in developing tools for a detailed characterisation of the humoral immune response in an NWM experimental animal model.

非人类灵长类动物 (NHP) 在现代生物医学研究中必不可少；新世界猴 (NWM) 主要用作人类疟疾的实验模型，因为它们提供有关寄生虫生物学和诱导免疫反应的有用信息。众所周知，候选疫苗的功效是由保护诱导抗体反应 (IgG) 介导的。

关于 IgG 亚类分子特征的信息不足，关于来自 Parvorder Platyrrhini 的 NHP。了解体液免疫反应的性质并表征 IgG 亚类的特征将提供有关使用 NHP 动物模型评估的疫苗免疫调节机制的宝贵信息。

本文基于免疫球蛋白重恒定γ (IGHG) 基因的CH1 至CH3 区的扩增、克隆和测序，对NWM 中的IgG 亚类（即Aotus、Cebus、Ateles和Alouatta 属）进行了表征。由此产生的序列能够阐明 IGHG 基因组织；在Aotus和Ateles猴群中发现了两个 IgG 变体，在Cebus和Alouatta中发现了三个 IgG 变体团体。这些序列在 Platyrrhini 中高度保守，并且具有与从 Parvorder Catarrrhini 中报道的猴子相似的结构。这些信息将有助于开发工具来详细表征 NWM 实验动物模型中的体液免疫反应。