Objective

This novel study explored the potential of two endolichenic fungi, Pseudopestalotiopsis theae (PT, accession number MG881833) and Astrocystis bambusae (AB, accession number MH370741) in decolorizing Malachite Green (MG).

Methods

Their efficacy in dye decolorization was compared to non-endolichenic fungus Trichoderma asperellum (TA, accession number KP792512). Decolorization test was performed using MG (100 ppm) for 14 days (absorbance at 617 nm) to determine decolorization efficiency (DE, %). The pellet was collected for FTIR analysis (4000 to 700 cm⁻¹, 50-scan speed) while the supernatant was analysed with UV-spectral analysis (300–800 nm) to detect for biodegradation.

Results

P. theae demonstrated the highest decolorization efficiency (DE%) at 89.22%, followed by T. asperellum (76.19%) and A. bambusae (67.69%). Common functional groups (i.e., OH, COOH, NH, CH, CO, CH) were detected in all isolates and their roles in biosorption were evident by the shifts in peaks and peak intensity. Biodegradation of MG was concluded based on UV-spectra peaks at 617 nm, which were significantly reduced (or absent).

Conclusion

This study is the first to reveal that endolichenic fungi P. theae and A. bambusae are capable of decolorizing the toxic dye malachite green.

中文翻译：

---

来自地衣松萝属的内生真菌对孔雀石绿染料的脱色：对其染料去除潜力的新研究

客观的

这项新颖的研究探索了两种内生真菌Pseudopestalotiopsis theae（PT，登录号 MG881833）和竹星囊藻（AB，登录号 MH370741）在孔雀石绿 (MG) 脱色方面的潜力。

方法

它们在染料脱色方面的功效与非内生真菌Trichoderma asperellum（TA，登录号 KP792512）进行了比较。使用 MG (100 ppm) 进行 14 天（617 nm 处的吸光度）的脱色测试以确定脱色效率（DE，%）。收集沉淀物用于 FTIR 分析（4000 至 700 cm ^-1，50 次扫描速度），同时用紫外光谱分析（300-800 nm）分析上清液以检测生物降解。

结果

P. theae表现出最高的脱色效率 (DE%)，为 89.22%，其次是T. asperellum (76.19%) 和A. bambusae (67.69%)。常见的官能团（即，OH，COOH，NH，CH，C O，Ç 2H）是在所有分离株以及它们在生物吸附作用检测通过在峰和峰强度的变化的是显而易见的。MG 的生物降解基于 617 nm 处的紫外光谱峰得出结论，该峰显着降低（或不存在）。

结论

这项研究首次揭示了内生真菌P. theae和A. bambusae能够使有毒染料孔雀石绿脱色。