Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2021-08-24 , DOI: 10.1007/s00216-021-03614-y Marc Kipping 1, 2 , Dirk Tänzler 1, 2 , Andrea Sinz 1, 2
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We describe a rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the direct detection and quantitation of SARS-CoV-2 nucleoprotein in gargle solutions and saliva. The method is based on a multiple-reaction monitoring (MRM) mass spectrometry approach with a total cycle time of 5 min per analysis and allows the detection and accurate quantitation of SARS-CoV-2 nucleoprotein as low as 500 amol/μL. We improved the sample preparation protocol of our recent piloting SARS-CoV-2 LC-MS study regarding sensitivity, reproducibility, and compatibility with a complementary reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) analysis of the same sample. The aim of this work is to promote diagnostic tools that allow identifying and monitoring SARS-CoV-2 infections by LC-MS/MS methods in a routine clinical environment.
Graphical abstract
中文翻译：
一种快速可靠的液相色谱/质谱分析方法，用于从漱口液和唾液中分析 SARS-CoV-2
我们描述了一种快速液相色谱/串联质谱 (LC-MS/MS) 方法，用于直接检测和定量漱口液和唾液中的 SARS-CoV-2 核蛋白。该方法基于多反应监测 (MRM) 质谱方法，每次分析的总循环时间为 5 分钟，可检测和准确定量低至 500 amol/μL 的 SARS-CoV-2 核蛋白。我们改进了我们最近试点 SARS-CoV-2 LC-MS 研究的样品制备方案，该研究涉及灵敏度、重现性和与同一样品的互补逆转录酶定量聚合酶链反应 (RT-qPCR) 分析的兼容性。这项工作的目的是推广诊断工具，允许在常规临床环境中通过 LC-MS/MS 方法识别和监测 SARS-CoV-2 感染。
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