'Cultured food' has tremendous potential as a sustainable meat alternative. Increased cultured food production is increasing the amount of waste medium from cell culture. Nitrogen- and phosphorus-containing compounds in waste medium can cause eutrophication of water bodies. Currently, microalgae are used in energy production, environmental protection, agriculture and pharmaceutical and health food industries. Here, we used the microalgae, Chlorococcum littorale and Chlorella vulgaris and the waste medium of C2C12 cells for a case study. We found that 80% and 26% of ammonia and 16% and 15% of phosphorus in the waste medium were consumed by C. littorale and C. vulgaris, respectively. In addition, C. littorale and C. vulgaris proliferated 3.2 folds and 1.6 folds, respectively, after seven days in the waste medium that was enhanced by adjusting medium salt concentration. This report demonstrates the potential of sustainability for solving the issue of waste medium production during the production of cultured food.

中文翻译：

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用于可持续“培养食品”生产的动物细胞废物培养基中的微藻培养。

“文化食品”作为一种可持续的肉类替代品具有巨大的潜力。培养食品产量的增加正在增加来自细胞培养的废物培养基的量。废弃介质中的含氮和磷化合物会导致水体富营养化。目前，微藻被用于能源生产、环保、农业和医药保健食品等行业。在这里，我们使用微藻、滨海绿球藻和普通小球藻以及 C2C12 细胞的废物培养基进行案例研究。我们发现废弃培养基中 80% 和 26% 的氨以及 16% 和 15% 的磷分别被 C. littorale 和 C. vulgaris 消耗。此外，C. littorale 和 C. vulgaris 分别增殖了 3.2 倍和 1.6 倍，在通过调整培养基盐浓度增强的废培养基中 7 天后。本报告展示了可持续性在解决培养食品生产过程中产生的废物培养基问题方面的潜力。