Carboxylation chitosan achieved by alkalization linked with ethyl vanillin to obtain Chitosan-ethyl vanillin (EV-CMCS) compound through Schiff base reaction and confirmed by FT-IR, UV, XRD, TG and NMR. EV-CMCS refluxed with GNRs for acquisition of EV-CMCS@GNRs nanocomposites for PTX Loading and release. Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL. It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%. Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8. It deduced that the broken Schiff base under acidic condition can increase CMC of EV-CMCS@GNRs, which offered an alternative way for paclitaxel delivery for tumor therapy.

羧化壳聚糖通过碱化与乙基香兰素连接，通过席夫碱反应得到壳聚糖-乙基香兰素（EV-CMCS）化合物，并经FT-IR、UV、XRD、TG和NMR证实。EV-CMCS 与 GNRs 回流以获取用于 PTX 加载和释放的 EV-CMCS@GNRs 纳米复合材料。结果表明，EV-CMCS 和 EV-CMCS@GNRs 都是纳米级复合材料，由于其胶束结构的 CMC 值为 0.06683 mg/mL 和 0.06537 mg/mL，因此具有优异的增溶性。发现 EV-CMCS 和 EV-CMCS@GNRs 对 PTX 的负载和封装率分别为 19.59~37.64% 和 60.36~80.79%，以及 20.99~37.02% 和 58.78~79.77%。与仅缓释EV-CMCS相比，它在pH 6.8和7.4下24小时累积释放量分别为11.5%和18.7%，48小时累积释放量分别为14.9%和23.7%，EV-CMCS@GNRs 代表突然释放，它在 pH 6.8 下 24 小时的累积释放量为 90.2%，48 小时的累积释放量为 96.0%。推断酸性条件下断裂的希夫碱可以增加EV-CMCS@GNRs的CMC，为紫杉醇递送用于肿瘤治疗提供了一种替代途径。