Cisplatin Inhibits Neurotransmitter Release during Exocytosis from Single Chromaffin Cells Monitored with Single Cell Amperometry,Electroanalysis

当前位置： X-MOL 学术 › Electroanalysis › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Cisplatin Inhibits Neurotransmitter Release during Exocytosis from Single Chromaffin Cells Monitored with Single Cell Amperometry
Electroanalysis ( IF 2.7 ) Pub Date : 2021-08-23 , DOI: 10.1002/elan.202100398
Xianchan Li ₁ , Mengying Wang ₁ , Yuying Liu ₁ , Jinchang Du ₁ , Junlan Zhou ₁ , Lijiao Cao ₁

Affiliation

Chemotherapy with cisplatin induces side effects such as memory loss, confusion of thinking, and difficulties with multi-tasking. However, the mechanism of cisplatin inducing nervous dysfunction is still unknown. Herein, we examine whether and how cisplatin regulates the release of neurotransmitter during exocytosis in single chromaffin cells using single cell amperometry. The results show that cisplatin reduces the amount of transmitter released during exocytosis by reducing the duration of the exocytotic events, including the opening and closing time of the fusion pore. Furthermore, the stability of the initial fusion pore formed during exocytosis is also reduced by cisplatin. Our study holds the promise for understanding the side effects of cisplatin on the nervous system at single cell level.

中文翻译：

顺铂抑制单细胞安培法监测的单嗜铬细胞胞吐过程中的神经递质释放

顺铂化疗会引起副作用，例如记忆力减退、思维混乱和多任务处理困难。然而，顺铂诱导神经功能障碍的机制仍不清楚。在这里，我们使用单细胞安培法检查顺铂是否以及如何在单嗜铬细胞胞吐过程中调节神经递质的释放。结果表明，顺铂通过减少胞吐事件的持续时间（包括融合孔的打开和关闭时间）来减少胞吐过程中释放的递质量。此外，在胞吐过程中形成的初始融合孔的稳定性也被顺铂降低。我们的研究有望在单细胞水平上了解顺铂对神经系统的副作用。

更新日期：2021-08-23

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11