Methodologies for Measuring Protein Trafficking across Cellular Membranes,ChemPlusChem

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Methodologies for Measuring Protein Trafficking across Cellular Membranes
ChemPlusChem ( IF 3.0 ) Pub Date : 2021-08-23 , DOI: 10.1002/cplu.202100304
Ziqi Lyu ₁ , Joseph C Genereux ₁

Affiliation

Nearly all proteins are synthesized in the cytosol. The majority of this proteome must be trafficked elsewhere, such as to membranes, to subcellular compartments, or outside of the cell. Proper trafficking of nascent protein is necessary for protein folding, maturation, quality control and cellular and organismal health. To better understand cellular biology, molecular and chemical technologies to properly characterize protein trafficking (and mistrafficking) have been developed and applied. Herein, we take a biochemical perspective to review technologies that enable spatial and temporal measurement of protein distribution, focusing on both the most widely adopted methodologies and exciting emerging approaches.

中文翻译：

跨细胞膜测量蛋白质转运的方法

几乎所有的蛋白质都是在胞质溶胶中合成的。这种蛋白质组的大部分必须被运输到其他地方，例如膜、亚细胞区室或细胞外。新生蛋白质的适当运输对于蛋白质折叠、成熟、质量控制以及细胞和有机体健康是必要的。为了更好地了解细胞生物学，已经开发和应用了正确表征蛋白质贩运（和非法贩运）的分子和化学技术。在这里，我们从生物化学的角度来回顾能够对蛋白质分布进行空间和时间测量的技术，重点关注最广泛采用的方法和令人兴奋的新兴方法。

更新日期：2021-10-12

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