Introduction: Obesity is a major risk factor for metabolic disorders in children. Therefore, it is particularly important to study the abnormal regulation of circulating miR-24-3p in obese children and its predictive value for metabolic syndrome. Methods: Serum samples were obtained from children with obesity (n = 45), obese children with metabolic syndrome (n = 52), and healthy controls (n = 50). The expression levels of miR-24-3p were detected by reverse transcription quantitative PCR. The ROC curve was used to evaluate the diagnostic value of miR-24-3p. Pearson’s correlation analysis was performed to evaluate the relationship between serum miR-24-3p and different clinical parameters. Logistic regression analysis was used to evaluate the relationship between miR-24-3p and obesity with metabolic syndrome in children. Results: The expression of miR-24-3p was the highest in obese children with metabolic syndrome. ROC results showed that miR-24-3p had the ability to distinguish healthy individuals from obese children (area under the curve [AUC] = 0.951) and can predict the occurrence of metabolic syndrome for obese children (AUC = 0.890). The expression level of miR-24-3p was positively correlated with body mass index (r = 0.817, p #x3c; 0.001), fasting blood glucose (r = 0.798, p #x3c; 0.001), triglycerides (r = 0.773, p #x3c; 0.001), systolic blood pressure (r = 0.746, p #x3c; 0.001), and diastolic blood pressure (r = 0.623, p #x3c; 0.001), respectively. Logistic regression analysis showed that miR-24-3p was an independent influence factor for the occurrence of metabolic syndrome in obese children. Discussion/Conclusion: MiR-24-3p is a potential noninvasive marker for children with obesity and has predictive value for the occurrence of metabolic syndrome.
Obes Facts

中文翻译：

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肥胖儿童循环miR-​​24-3p的失调及其对代谢综合征的预测价值

简介：肥胖是儿童代谢紊乱的主要危险因素。因此，研究肥胖儿童循环miR-​​24-3p的异常调控及其对代谢综合征的预测价值显得尤为重要。方法：从肥胖儿童（ n = 45）、代谢综合征肥胖儿童（ n = 52）和健康对照（ n= 50)。通过逆转录定量PCR检测miR-24-3p的表达水平。ROC曲线用于评价miR-24-3p的诊断价值。进行 Pearson 相关分析以评估血清 miR-24-3p 与不同临床参数之间的关系。采用Logistic回归分析评估miR-24-3p与儿童代谢综合征肥胖的关系。结果：miR-24-3p的表达在肥胖代谢综合征儿童中最高。ROC结果显示，miR-24-3p具有区分健康个体和肥胖儿童的能力（曲线下面积[AUC] = 0.951），可以预测肥胖儿童代谢综合征的发生（AUC = 0.890）。miR-24-3p的表达水平与体重指数（r =0.817，p #x3c；0.001）、空腹血糖（r =0.798，p #x3c；0.001）、甘油三酯（r =0.773，p #x3c; 0.001)、收缩压 ( r = 0.746, p #x3c; 0.001) 和舒张压 ( r = 0.623,p #x3c; 0.001），分别。Logistic回归分析显示miR-24-3p是肥胖儿童代谢综合征发生的独立影响因素。讨论/结论： MiR-24-3p是肥胖儿童潜在的无创标志物，对代谢综合征的发生具有预测价值。
肥胖事实