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A complex regulatory network underlies de novo root regeneration in red maple (Acer rubrum)
Botany ( IF 1.0 ) Pub Date : 2021-04-23 , DOI: 10.1139/cjb-2020-0199
Mengyi Li 1 , Yunhe Cheng 2 , Yanlin Li 3 , Dexin Gan 3 , Xiaoying Yu 3
Affiliation  

Botany, Ahead of Print.
Red maple (Acer rubrum L.) is ornamentally and medicinally valuable; however, its wider application is restricted by the difficulty of rooting from cuttings. We analyzed paraffin sections of roots regenerating using RNA-Seq to decipher the mechanisms underlying de novo root regeneration (DNRR) in red maple cuttings. This work contributes to improving the rooting rate and shortening the rooting time. We identified four stages during DNRR: 0 days after induction (DAI), no new cell formation; 30 DAI, root meristem organization; 36 DAI, root primordium formation; and 45 DAI, root elongation growth. We identified 37 959 unigenes by de novo assembly, with 25 477 (67.12%) functionally annotated. Furthermore, we identified 1285 differentially expressed genes (DEGs) between adjacent stages. From Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment networks, we found evidence that plant hormones are significant in DNRR of red maple cuttings. Specifically, 149 DEGs functioned in hormone signal transduction pathways, particularly those involving ethylene, auxin, and jasmonic acid (JA). We propose a complex regulatory network model of DNRR in red maple, where wounding induces root regeneration through pathways of JA and auxin signaling. The transcription factors ETHYLENE RESPONSE FACTOR 109 (ERF109) and ERF115 integrate JA signal and participate in DNRR directly by regulating SCARECROW activation and indirectly by promoting auxin biosynthesis.


中文翻译:

一个复杂的调控网络是红枫(Acer rubrum)从头再生根的基础

植物学,超前印刷。
红枫 (Acer rubrum L.) 具有观赏和药用价值;然而,其更广泛的应用受到扦插生根困难的限制。我们使用 RNA-Seq 分析了根再生的石蜡切片,以破译红枫扦插从头再生 (DNRR) 的机制。这项工作有助于提高生根率和缩短生根时间。我们在 DNRR 期间确定了四个阶段:诱导后 0 天 (DAI),没有新细胞形成;30 DAI,根分生组织;36 DAI,根原基形成;和 45 DAI,根伸长生长。我们通过从头组装鉴定了 37 959 个 unigene,其中 25 477 (67.12%) 个进行了功能注释。此外,我们确定了相邻阶段之间的 1285 个差异表达基因 (DEG)。从基因本体论和京都基因和基因组富集网络百科全书,我们发现了植物激素在红枫插条的 DNRR 中显着的证据。具体而言,149 个 DEG 在激素信号转导通路中起作用,尤其是那些涉及乙烯、生长素和茉莉酸 (JA) 的通路。我们提出了一个复杂的红枫 DNRR 调控网络模型,其中伤口通过 JA 和生长素信号传导途径诱导根再生。转录因子 ETHYLENE RESPONSE FACTOR 109 (ERF109) 和 ERF115 整合 JA 信号并通过调节 SCARECROW 激活直接参与 DNRR,并通过促进生长素生物合成间接参与。特别是涉及乙烯、生长素和茉莉酸 (JA) 的那些。我们提出了一个复杂的红枫 DNRR 调控网络模型,其中伤口通过 JA 和生长素信号通路诱导根再生。转录因子 ETHYLENE RESPONSE FACTOR 109 (ERF109) 和 ERF115 整合 JA 信号并通过调节 SCARECROW 激活直接参与 DNRR,并通过促进生长素生物合成间接参与。特别是涉及乙烯、生长素和茉莉酸 (JA) 的那些。我们提出了一个复杂的红枫 DNRR 调控网络模型,其中伤口通过 JA 和生长素信号通路诱导根再生。转录因子 ETHYLENE RESPONSE FACTOR 109 (ERF109) 和 ERF115 整合 JA 信号并通过调节 SCARECROW 激活直接参与 DNRR,并通过促进生长素生物合成间接参与。
更新日期:2021-04-23
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