Enzymatic catalysis is a highly attractive approach to the DNA encoded library technology (DEL) that has not been widely explored. In this paper, we report an l-threonine aldolase (l-TA)-catalyzed on-DNA aldol reaction to form β-hydroxy-α-amino acids, and its diastereoselectivity determination. l-TAs from three species show good on-DNA aldehyde scope and complementary stereoselectivity. The formed aldol product can be further diversified via various reactions, which demonstrates the utility of this reaction in DEL.

中文翻译：

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L-苏氨酸醛缩酶在 DNA 反应中的应用

酶催化是一种极具吸引力的 DNA 编码文库技术 (DEL)，但尚未得到广泛探索。在本文中，我们报告了l-苏氨酸醛缩酶 ( l -TA) 催化的 DNA 醛醇反应形成 β-羟基-α-氨基酸，及其非对映选择性测定。来自三个物种的l -TAs 显示出良好的 DNA 醛范围和互补的立体选择性。形成的羟醛产物可以通过各种反应进一步多样化，这证明了该反应在 DEL 中的实用性。