Abstract

Candida albicans causes candidiasis, particularly in immunocompromised patients. Streptococcus salivarius K12 (K12) is a probiotic isolated from a healthy oral cavity. The study aimed to determine the effect of K12 on C. albicans aggregation, biofilm formation and dimorphism. C. albicans ATCC MYA-4901, acquired immunodeficiency syndrome (AIDS) isolate (ALC2), and oral cancer isolate (ALC3) and K12 were used in the study. All C. albicans strains and K12 were grown in yeast peptone dextrose agar and brain heart infusion agar, respectively, prior to aggregation, biofilm and dimorphism assays. Auto-aggregation of C. albicans MYA-4901 and ALC2 was categorised as high, while the co-aggregation of the strains was low in the presence of K12. C. albicans total cell count decreased significantly when co-cultured with K12 compared with monocultured C. albicans biofilm (p < 0.05). Inhibition of yeast-to-hyphae transition was also observed when co-cultured with K12. In conclusion, K12 inhibits C. albicans aggregation, biofilm formation and dimorphism.

摘要

白色念珠菌会引起念珠菌病，尤其是在免疫功能低下的患者中。唾液链球菌K12 (K12) 是一种从健康口腔中分离出来的益生菌。该研究旨在确定 K12 对白色念珠菌聚集、生物膜形成和二态性的影响。研究中使用了白色念珠菌ATCC MYA-4901、获得性免疫缺陷综合征 (AIDS) 分离株 (ALC2) 和口腔癌分离株 (ALC3) 和 K12。在聚集、生物膜和二态性分析之前，所有白色念珠菌菌株和 K12 分别在酵母蛋白胨葡萄糖琼脂和脑心浸液琼脂中生长。白色念珠菌的自动聚集MYA-4901 和 ALC2 被归类为高，而在 K12 存在下菌株的共聚集性较低。与单一培养的白色念珠菌生物膜相比，当与 K12 共培养时，白色念珠菌的总细胞数显着减少( p <  0.05)。当与 K12 共培养时，还观察到酵母菌向菌丝转变的抑制作用。总之，K12 抑制白色念珠菌聚集、生物膜形成和二态性。