Uterine tissue engineering can provide the opportunity for curing female infertility. Natural scaffold is a good choice to recapitulate the architecture and functionality of the native tissue. In this study, we purposed the potential of uterine decellularized scaffolds as an adequate natural niche for MenSCs differentiation toward uterus-specific cell lineages. Mouse’s uterus was decellularized by immersion of hypo and hypertonic salts or freeze–thaw cycle followed by immersion in Triton X-100 and SDS solutions. MenSCs were isolated from the menstrual blood of 6 healthy women. The decellularized and recellularized samples were prepared for further in vitro and in vivo analyses. Histochemical studies and Raman spectroscopy revealed uterine ECM was preserved well, and the cells were completely removed after decellularization. Scanning electron microscopy (SEM) showed that the 3D ultrastructure of the uterus remained intact. Flowcytometric examination with CD34, CD44, CD105, CD106, CD144 markers revealed stem cell characters of cells that isolated from menstrual blood. MTT assay confirmed the bioavailability of MenSCs cultured scaffolds after 7 and 10 days. Histochemical studies, SEM images, and Raman spectra showed MenSCs seeded and growth in uterine scaffolds. Immunostaining using anti-cytokeratin (CK), anti-desmin (Des), anti-vimentin (Vim), and anti-smooth muscle actin (SMA) antibodies showed MenSCs differentiation to epithelial and smooth muscle tissues. The Raman spectroscopy revealed the extracellular matrix (ECM) of decellularized uterine scaffolds was well preserved. The decellularized uterus can be considered a promising vehicle to support cell transplantation and differentiation. MenSCs are a good choice for uterine tissue engineering. The complete decellularization from mice uterine tissue was done by combining chemical agents

中文翻译：

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使用经血干细胞 (MenSCs) 和脱细胞子宫支架对小鼠子宫进行组织工程

子宫组织工程可以为治疗女性不孕症提供机会。天然支架是重现天然组织结构和功能的不错选择。在这项研究中，我们旨在将子宫脱细胞支架作为 MenSCs 向子宫特异性细胞谱系分化的适当天然生态位的潜力。通过浸泡低渗和高渗盐或冻融循环，然后浸泡在 Triton X-100 和 SDS 溶液中，使小鼠的子宫脱细胞。从 6 名健康女性的经血中分离出 MenSCs。制备脱细胞和再细胞化样品用于进一步的体外和体内分析。组织化学研究和拉曼光谱显示，子宫 ECM 保存良好，脱细胞后细胞完全去除。扫描电子显微镜 (SEM) 显示子宫的 3D 超微结构保持完整。用 CD34、CD44、CD105、CD106、CD144 标志物进行流式细胞术检查显示从月经血中分离的细胞的干细胞特征。MTT 测定证实了 MenSCs 培养支架在 7 天和 10 天后的生物利用度。组织化学研究、SEM 图像和拉曼光谱显示 MenSCs 在子宫支架中播种和生长。使用抗细胞角蛋白 (CK)、抗结蛋白 (Des)、抗波形蛋白 (Vim) 和抗平滑肌肌动蛋白 (SMA) 抗体的免疫染色显示 MenSCs 分化为上皮和平滑肌组织。拉曼光谱显示脱细胞子宫支架的细胞外基质 (ECM) 保存完好。去细胞化的子宫可以被认为是支持细胞移植和分化的有前途的载体。MenSCs 是子宫组织工程的不错选择。通过结合化学试剂完成小鼠子宫组织的完全脱细胞