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Correction to: Human microbiota modulation via QseC sensor kinase mediated in the Escherichia coli O104:H4 outbreak strain infection in microbiome model
BMC Microbiology ( IF 4.2 ) Pub Date : 2021-08-24 , DOI: 10.1186/s12866-021-02266-3
Tamara Renata Machado Ribeiro 1 , Mateus Kawata Salgaço 2 , Maria Angela Tallarico Adorno 3 , Miriam Aparecida da Silva 4 , Roxane Maria Fontes Piazza 4 , Katia Sivieri 2 , Cristiano Gallina Moreira 1
Affiliation  

Correction to: BMC Microbiology (2021) 21:163

https://doi.org/10.1186/s12866-021-02220-3

Following the publication of the original article [1], we were notified that the captions for Figs. 2, 3 and 5 needed adjustments.

Original captions:

  • Fig. 2: “Microbiota predominance modulated via QseC during C227–11 infection in the SHIME® model. Relative microbiota abundance analysis via qRT-PCR of 16 s rRNA of phyla and genera. Microbiota composition from days 0 to 3 p.i with strain C227–11 infection, respectively, phyla and genera (a and b), and with strain C227–11::qseC infection, respectively, phyla and genera (c and d). ELISA Immunoassay capture to measure the Stx levels from the output collected during the SHIME® infection, day 1, ** p = 0.002 and 3 p.i., ** p = 0.009 (e). The statistical significance analyzes were performed on GraphPad Prism 7 via t-test”

  • Fig. 3: “Direct acetate, propionate and butyrate production analysis (mmol/L) from day 0 to day 3.p.i. via gas chromatography. SCFA composition from C227–11 infection period (a) (*** p = 0.0003) and C227–11::qseC (b). Analyzes were performed individually for each SCFA compared to day 0. The statistical significance analyzes were performed on GraphPad Prism 7 via one-way ANOVA and Tukey post hoc test (*p = 0.0371, *p = 0.0309, *** p = 0.0001)”

  • Fig. 5: “Microbiota predominance during C57BL/6 mice infection, C227–11and C227–11::qseC strains (a). Expression levels of qseC during early and later infection (day 1-3p.i.) of C227–11, 042 and DH5α strains, p-values are respectively p =0.006 (**), p = 0.001 (**) and p = 0.004 (**) (b). Relative expression levels were measured in vitro of stx2a gene from the C227–11, C227–11::qseC, and C227–11qseC+ (pBAD33 qseC), p = 0.01 (**), p = 0.001 (***) (c)”

Corrected captions:

  • Fig. 2: “Microbiota predominance modulated via QseC during C227–11 infection in the SHIME® model. Relative microbiota abundance analysis via qRT-PCR of 16 s rRNA of phyla and genera. Microbiota composition from days 0 to 3 p.i with strain C227–11 infection, respectively, phyla and genera (a and b), and with strain C227– 11::qseC infection, respectively, phyla and genera (c and d). ELISA Immunoassay capture to measure the Stx levels from the output collected during the SHIME® infection, day 1, ** p = 0.002 and 3 p.i., ** p = 0.009 (e). The statistical significance analyzes were performed on GraphPad Prism 7 via t-test”

  • Fig. 3: “Direct acetate, propionate and butyrate production analysis (mmol/L) from day 0 to day 3.p.i. via gas chromatography. SCFA composition from C227–11 infection period (a) (*** p = 0.0003) and C227–11::qseC (b). Analyzes were performed individually for each SCFA compared to day 0. The statistical significance analyzes were performed on GraphPad Prism 7 via one-way ANOVA and Tukey post hoc test (*p = 0.0371, *p = 0.0309, *** p = 0.0001)”

  • “Fig. 5: Microbiota predominance during C57BL/6 mice infection, C227–11and C227–11::qseC strains (a). Expression levels of qseC during early and later infection (day 1-3p.i.) of C227–11, 042 and DH5α strains, p-values are respectively p =0.006 (**), p = 0.001 (**) and p = 0.004 (**) (b). Relative expression levels were measured in vitro of stx2a gene from the C227–11, C227–11::qseC, and C227–11qseC+ (pBAD33 qseC), p = 0.01 (**), p = 0.001 (***) (c)”

The original article has been corrected.

  1. 1.

    Ribeiro M, et al. Human microbiota modulation via QseC sensor kinase mediated in the Escherichia coli O104:H4 outbreak strain infection in microbiome model (2021) 21:163. 2021;21(1):163. https://doi.org/10.1186/s12866-021-02220-3.

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Affiliations

  1. Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, SP, Brazil

    Tamara Renata Machado Ribeiro & Cristiano Gallina Moreira

  2. Department of Food and Nutrition, School of Pharmaceutical Sciences, São Paulo State University, (UNESP), Araraquara, SP, Brazil

    Mateus Kawata Salgaço & Katia Sivieri

  3. Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo (USP), São Carlos, SP, Brazil

    Maria Angela Tallarico Adorno

  4. Bacteriology Laboratoty, Butantan Institute, São Paulo, SP, Brazil

    Miriam Aparecida da Silva & Roxane Maria Fontes Piazza

Authors
  1. Tamara Renata Machado RibeiroView author publications

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  2. Mateus Kawata SalgaçoView author publications

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  3. Maria Angela Tallarico AdornoView author publications

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  4. Miriam Aparecida da SilvaView author publications

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  5. Roxane Maria Fontes PiazzaView author publications

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  6. Katia SivieriView author publications

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  7. Cristiano Gallina MoreiraView author publications

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Corresponding author

Correspondence to Cristiano Gallina Moreira.

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Ribeiro, T.R.M., Salgaço, M.K., Adorno, M.A.T. et al. Correction to: Human microbiota modulation via QseC sensor kinase mediated in the Escherichia coli O104:H4 outbreak strain infection in microbiome model. BMC Microbiol 21, 233 (2021). https://doi.org/10.1186/s12866-021-02266-3

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  • DOI: https://doi.org/10.1186/s12866-021-02266-3

中文翻译：
更正：通过 QseC 传感器激酶在微生物组模型中大肠杆菌 O104:H4 暴发菌株感染介导的人类微生物群调节

更正：BMC Microbiology (2021) 21:163

https://doi.org/10.1186/s12866-021-02220-3

在原始文章 [1] 发表后，我们被告知无花果的标题。2、3 和 5 需要调整。

原文字幕：

  • 图 2：“SHIME® 模型中 C227-11 感染期间通过 QseC 调节的微生物群优势。通过门和属的 16 s rRNA 的 qRT-PCR 进行相对微生物群丰度分析。感染后第 0 天至第 3 天的微生物群组成，分别感染 C227-11 菌株，门和属（a 和 b），以及菌株 C227-11::qseC 感染，分别感染门和属（c 和 d）。ELISA 免疫测定捕获以测量感染期间收集的输出中的 Stx 水平，第 1 天，** p = 0.002 和 3 pi，** p = 0.009 (e)。通过 t 检验在 GraphPad Prism 7 上进行统计显着性分析”

  • 图 3：“通过气相色谱法从第 0 天到第 3 天的直接乙酸盐、丙酸盐和丁酸盐生产分析 (mmol/L)。来自 C227-11 感染期 (a) (*** p = 0.0003) 和 C227-11::qseC (b) 的 SCFA 组成。与第 0 天相比，对每个 SCFA 单独进行分析。通过单向方差分析和 Tukey 事后检验对 GraphPad Prism 7 进行统计显着性分析（*p = 0.0371，*p = 0.0309，*** p = 0.0001） ”

  • 图 5：“C57BL/6 小鼠感染期间微生物群的优势，C227-11 和 C227-11::qseC 菌株（a）。在 C227-11、042 和 DH5α 菌株的早期和后期感染（第 1-3 天）期间 qseC 的表达水平，p 值分别为 p = 0.006 (**)、p = 0.001 (**) 和 p = 0.004 (**) (b)。在体外测量了来自 C227-11、C227-11::qseC 和 C227-11qseC+ (pBAD33 qseC) 的 stx2a 基因的相对表达水平，p = 0.01 (**)，p = 0.001 (***) (c )”

更正的字幕：

  • 图 2：“SHIME® 模型中 C227-11 感染期间通过 QseC 调节的微生物群优势。通过门和属的 16 s rRNA 的 qRT-PCR 进行相对微生物群丰度分析。感染后第 0 天至第 3 天的微生物群组成，分别感染 C227-11 菌株，门和属（a 和 b），以及菌株 C227-11::qseC 感染，分别感染门和属（c 和 d）。ELISA 免疫测定捕获以测量感染期间收集的输出中的 Stx 水平，第 1 天，** p = 0.002 和 3 pi，** p = 0.009 (e)。通过 t 检验在 GraphPad Prism 7 上进行统计显着性分析”

  • 图 3：“通过气相色谱法从第 0 天到第 3 天的直接乙酸盐、丙酸盐和丁酸盐生产分析 (mmol/L)。来自 C227-11 感染期 (a) (*** p = 0.0003) 和 C227-11::qseC (b) 的 SCFA 组成。与第 0 天相比，对每个 SCFA 单独进行分析。通过单向方差分析和 Tukey 事后检验对 GraphPad Prism 7 进行统计显着性分析（*p = 0.0371，*p = 0.0309，*** p = 0.0001） ”

  • “无花果。图 5：C57BL/6 小鼠感染期间微生物群的优势，C227-11 和 C227-11::qseC 菌株（a）。在 C227-11、042 和 DH5α 菌株的早期和后期感染（第 1-3 天）期间 qseC 的表达水平，p 值分别为 p = 0.006 (**)、p = 0.001 (**) 和 p = 0.004 (**) (b)。在体外测量了来自 C227-11、C227-11::qseC 和 C227-11qseC+ (pBAD33 qseC) 的 stx2a 基因的相对表达水平，p = 0.01 (**)，p = 0.001 (***) (c )”

原文章已更正。

  1. 1.

    里贝罗 M，等。通过 QseC 传感器激酶调节人类微生物群在微生物组模型中的大肠杆菌 O104:H4 爆发株感染中介导 (2021) 21:163。2021;21(1):163。https://doi.org/10.1186/s12866-021-02220-3。

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隶属关系

  1. 生物科学系，药学院，圣保罗州立大学 (UNESP)，阿拉拉夸拉，SP，巴西

    塔玛拉·雷纳塔·马查多·里贝罗和克里斯蒂亚诺·加利纳·莫雷拉

  2. 巴西圣保罗州立大学（UNESP）药学院食品与营养系，阿拉拉夸拉，SP

    Mateus Kawata Salgaço & Katia Sivieri

  3. 水力学和卫生系，圣卡洛斯工程学院，圣保罗大学 (USP)，巴西圣卡洛斯

    玛丽亚·安吉拉·塔拉里科·阿多诺

  4. 细菌学实验室，布坦坦研究所，圣保罗，SP，巴西

    Miriam Aparecida da Silva & Roxane Maria Fontes Piazza

作者
  1. Tamara Renata Machado Ribeiro查看作者出版物

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  2. Mateus Kawata Salgaço查看作者出版物

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  3. Maria Angela Tallarico Adorno查看作者出版物

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  4. Miriam Aparecida da Silva查看作者出版物

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  5. Roxane Maria Fontes Piazza查看作者出版物

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  6. Katia Sivieri查看作者出版物

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  7. Cristiano Gallina Moreira查看作者出版物

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通讯作者

致克里斯蒂亚诺·加利纳·莫雷拉 (Cristiano Gallina Moreira) 的信件。

开放获取本文根据知识共享署名 4.0 国际许可协议获得许可，该许可允许以任何媒体或格式使用、共享、改编、分发和复制，只要您适当注明原作者和来源，提供链接到知识共享许可，并指出是否进行了更改。本文中的图像或其他第三方材料包含在文章的知识共享许可中，除非在材料的信用额度中另有说明。如果文章的知识共享许可中未包含材料，并且您的预期用途未得到法律法规的允许或超出允许的用途，则您需要直接从版权所有者处获得许可。要查看此许可证的副本，请访问 http://creativecommons.org/licenses/by/4.0/。

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引用这篇文章

Ribeiro、TRM、Salgaço、MK、Adorno、MAT等。更正：通过 QseC 传感器激酶在微生物组模型中大肠杆菌O104:H4 爆发株感染中介导的人类微生物群调节。BMC 微生物学 21, 233 (2021)。https://doi.org/10.1186/s12866-021-02266-3

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  • 发表：

  • DOI : https://doi.org/10.1186/s12866-021-02266-3
更新日期：2021-08-24
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