Abstract

Proteins MSL1 and MSL2 form the core of the Drosophila dosage compensation complex, which specifically binds to the X chromosome of males. Phosphorylation of certain amino acid residues was previously shown to regulate MSL1 activity. In the present work, transgenic lines of Drosophila expressing mutant variants of the MSL1 protein were obtained, in which amino acids undergoing phosphorylation were replaced. As a result, it was shown that inactivation of phosphorylation sites does not affect the efficiency of specific binding of the dosage compensation complex to the X chromosome of males and its functional activity.

中文翻译：

---

MSL1 蛋白磷酸化位点的突变不影响果蝇的剂量补偿

 抽象的

蛋白质 MSL1 和 MSL2 形成果蝇剂量补偿复合物的核心，专门与雄性 X 染色体结合。先前已证明某些氨基酸残基的磷酸化可以调节 MSL1 活性。在目前的工作中，获得了表达MSL1蛋白突变体的果蝇转基因系，其中经历磷酸化的氨基酸被替换。结果表明，磷酸化位点的失活并不影响剂量补偿复合物与男性X染色体特异性结合的效率及其功能活性。