Abstract

Occupational and environmental exposure to chromium compounds leads to nephrotoxicity to humans and animals due to the overproduction of ROS. Our study was aimed to demonstrate the shielding effect of hydroethanolic extract of Ipomoea staphylina (HEIS) bark on male Wistar rats challenged with potassium dichromate (K₂Cr₂O₇). Division of animals was done in 4 groups’ viz., normal control, K₂Cr₂O₇ control, K₂Cr₂O₇+HEIS (100 mg/kg), and K₂Cr₂O₇+HEIS (200 mg/kg). Except for the normal control group, other groups were challenged with a single dose (subcutaneous) of K₂Cr₂O₇ (15 mg/kg) and then treated with HEIS (100 and 200 mg/kg) for 1 week. It was observed that animals treated with K₂Cr₂O₇ showed a notable increase in serum creatinine, blood urea, and BUN and dwindles in protein level. These changes were significantly reversed after a 1-week treatment with HEIS (100 and 200 mg/kg). Moreover, HEIS (100 and 200 mg/kg) showed a remarkable improvement in the activity of antioxidant enzymes (GPx, CAT, and SOD) and decreased the levels of TNF-α and IL-1β in the kidney. Furthermore, treatment with HEIS (100 and 200 mg/kg) notably decreased the activity of caspase-3 and improved the level of HO-1 especially in the K₂Cr₂O₇+ HEIS (200 mg/kg) group. Also, the histopathological study of the kidney supported the protective effects of HEIS. Hence, HEIS bark holds a notable protective effect against K₂Cr₂O₇-induced nephrotoxicity in rats.

中文翻译：

---

Ipomoea staphylina 通过抗氧化和抗细胞凋亡作用减轻 Wistar 大鼠重铬酸钾诱导的肾毒性

 抽象的

职业和环境接触铬化合物会由于活性氧过量产生而对人类和动物产生肾毒性。我们的研究旨在证明葡萄藤树皮氢乙醇提取物 (HEIS) 对受到重铬酸钾 (K ₂ Cr ₂ O ₇ ) 攻击的雄性 Wistar 大鼠的屏蔽作用。将动物分为4组，即正常对照、K ₂ Cr ₂ O ₇对照、K ₂ Cr ₂ O ₇ +HEIS (100 mg/kg)和K ₂ Cr ₂ O ₇ +HEIS (200 mg/kg)。 /千克）。除正常对照组外，其他组均采用单剂量（皮下）K ₂ Cr ₂ O ₇ （15 mg/kg）攻击，然后用HEIS（100和200 mg/kg）治疗1周。据观察，用K ₂ Cr ₂ O ₇治疗的动物表现出血清肌酐、血尿素和BUN显着增加，而蛋白质水平下降。经过 1 周 HEIS（100 和 200 mg/kg）治疗后，这些变化显着逆转。此外，HEIS（100 和 200 mg/kg）显着提高了抗氧化酶（GPx、CAT 和 SOD）的活性，并降低了肾脏中 TNF-α 和 IL-1β 的水平。此外，HEIS（100和200 mg/kg）处理显着降低了caspase-3的活性并提高了HO-1的水平，特别是在K ₂ Cr ₂ O ₇ + HEIS（200 mg/kg）组中。此外，肾脏的组织病理学研究也支持了 HEIS 的保护作用。 因此，HEIS树皮对K ₂ Cr ₂ O ₇诱导的大鼠肾毒性具有显着的保护作用。