Antibody drugs are one of the most important therapeutic modalities in cancer treatment. Antibody drugs for canine cytotoxicity have been developed, and the most important way to evaluate the function of antibody drugs in vitro is to measure the antibody-dependent cellular cytotoxicity (ADCC) activity. However, a good method for measuring the ADCC activity of antibody drugs for dogs has not yet been developed. This study generated a cell line of NK-92 cells, a human natural killer cell line, transfected with canine CD16 and γ-chain as effector cells. A20 cells expressing canine CD20 were used as target cells, and a system for ADCC activity was established using an anti-dog CD20 antibody. The assay generated very consistent results. This is an applicable method for measuring canine ADCC activity and can be used for various antigen-antibody combinations to develop canine antibody drugs with cytotoxic activity in the future.

抗体药物是癌症治疗中最重要的治疗方式之一。已经开发出针对犬细胞毒性的抗体药物，而在体外评价抗体药物功能的最重要方法是测量抗体依赖性细胞毒性（ADCC）活性。然而，尚未开发出一种用于测量犬用抗体药物的ADCC活性的好方法。这项研究产生了一个由犬类 CD16 和 γ 链作为效应细胞转染的 NK-92 细胞系，一种人类自然杀伤细胞系。使用表达犬CD20的A20细胞作为靶细胞，并使用抗狗CD20抗体建立ADCC活性系统。该测定产生了非常一致的结果。