Objective: To study the bone induction and defect repair of true bone ceramics (TBC) combined with rhBMP-2 and Sr. Methods: MC3T3-E1 cells were used to evaluate the bioactivity of the composite. Cell proliferation activity was detected by CCK-8, ALP activity was detected by p-nitrophenyl phosphate (PNPP), and the differences of material surface topography were observed by scanning electron microscopy (SEM). Bone induction was verified by the implantation in nude mice. The rabbit femoral condyle defect model was achieved to verify the bone defect repair ability of the material. Results: SEM results showed nearly the same surface morphology and cell proliferation quantified by CCK-8 showed that compared with TBC, both TBC&Sr and TBC&BMP-2&Sr had a significant promoting effect (P < 0.05). ALP activity result showed that the ALP activity of TBC&BMP-2&Sr was significantly higher than that of TBC alone (P < 0.05). The bone induction result showed that TBC&Sr had a small amount of new bone formation, and the new bone area was only 2.5 ± 0.11%. The bone induction activity of TBC&BMP-2&Sr was the highest, the new bone area was up to 75.36 ± 4.21%. Histological result of bone defect repair showed that TBC&BMP-2&Sr was also the highest, the new bone area was up to 72.42 ± 3.14%. The repair effect of TBC& BMP-2 was second, and better than that of TBC&Sr. Conclusion: TBC combined with rhBMP-2 and Sr had the good bioactivity, obvious bone conduction and bone defect repair performance, laying the foundation of clinical application potentially.

目的：研究真骨陶瓷（TBC）联合rhBMP-2和Sr的骨诱导和缺损修复。方法：采用MC3T3-E1细胞评价该复合材料的生物活性。CCK-8检测细胞增殖活性，磷酸对硝基苯酯(PNPP)检测ALP活性，扫描电镜(SEM)观察材料表面形貌的差异。通过植入裸鼠来验证骨诱导。建立兔股骨髁缺损模型，验证材料的骨缺损修复能力。结果： SEM结果显示CCK-8量化的细胞表面形态和细胞增殖几乎相同，表明与TBC相比，TBC&Sr和TBC&BMP-2&Sr均具有显着的促进作用（P < 0.05)。ALP活性结果显示，TBC&BMP-2&Sr的ALP活性显着高于单独使用TBC的( P  < 0.05)。骨诱导结果显示TBC&Sr有少量新骨形成，新骨面积仅为2.5±0.11%。TBC&BMP-2&Sr的骨诱导活性最高，新骨面积达75.36±4.21%。骨缺损修复组织学结果显示TBC&BMP-2&Sr也最高，新骨面积达72.42±3.14%。TBC&BMP-2修复效果次之，优于TBC&Sr。结论：TBC联合rhBMP-2和Sr具有良好的生物活性，具有明显的骨传导和骨缺损修复性能，可能为临床应用奠定基础。