Combination of bioactive material such as hydroxyapatite (HAp) with antibacterial agents would have great potential to be used as bone implant materials to avert possible bacterial infection that can lead to implant-associated diseases. The present study aimed to develop an antibacterial silver nanoparticle-decorated hydroxyapatite (HAp/AgNPs) nanocomposite using chemical reduction and thermal calcination approaches. In this work, natural HAp that was extracted from chicken bone wastes is used as support matrix for the deposition of silver nanoparticles (AgNPs) to produce HAp/AgNPs nanocomposite. XRD, FESEM-EDX, HRTEM, and XPS analyses confirmed that spherical AgNPs were successfully synthesized and deposited on the surface of HAp particles, and the amount of AgNPs adhered on the HAp surface increased with increasing AgNO₃ concentration used. The synthesized HAp/AgNPs nanocomposites demonstrated strong antibacterial activity against Staphylococcus aureus bacteria, where the antibacterial efficiency is relied on the amount and size of deposited AgNPs. In addition, the in vitro bioactivity examination in Hank’s balanced salt solution showed that more apatite were grown on the surface of HAp/AgNPs nanocomposite when AgNO₃ concentration used >1 wt.%. Such nanocomposite with enhanced bioactivity and antibacterial properties emerged as a promising biomaterial to be applied for dentistry and orthopedic implantology.

羟基磷灰石 (HAp) 等生物活性材料与抗菌剂的组合具有很大的潜力，可用作骨植入材料，以避免可能导致植入相关疾病的细菌感染。本研究旨在利用化学还原和热煅烧方法开发一种抗菌银纳米颗粒修饰的羟基磷灰石 (HAp/AgNPs) 纳米复合材料。在这项工作中，从鸡骨废料中提取的天然 HAp 被用作沉积银纳米颗粒 (AgNPs) 的支撑基质，以生产 HAp/AgNPs 纳米复合材料。XRD、FESEM-EDX、HRTEM和XPS分析证实，球形AgNPs成功合成并沉积在HAp颗粒表面，且附着在HAp表面的AgNPs数量随着AgNO _{3的增加而增加}使用的浓度。合成的 HAp/AgNPs 纳米复合材料对金黄色葡萄球菌具有很强的抗菌活性，其抗菌效率取决于沉积的 AgNPs 的数量和大小。此外，Hank 平衡盐溶液中的体外生物活性检测表明，当 AgNO ₃浓度大于 1 wt.% 时，更多的磷灰石在 HAp/AgNPs 纳米复合材料表面生长。这种具有增强的生物活性和抗菌特性的纳米复合材料成为一种很有前途的生物材料，可应用于牙科和骨科植入学。