Elucidating key plant growth–promoting (PGPR) traits in Burkholderia sp. Nafp2/4-1b (=SARCC-3049) using gnotobiotic assays and whole-genome-sequence analysis,Letters in Applied Microbiology

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Elucidating key plant growth–promoting (PGPR) traits in Burkholderia sp. Nafp2/4-1b (=SARCC-3049) using gnotobiotic assays and whole-genome-sequence analysis
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2021-08-24 , DOI: 10.1111/lam.13556
A I Hassen ₁ , L S Khambani ₂ , Z H Swanevelder ₃ , N P Mtsweni ₁ , F L Bopape ₁ , A van Vuuren ₁ , E J van der Linde ₁ , L Morey ₄

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Burkholderia sp. Nafp2/4-1b (=SARCC-3049) is a plant growth–promoting rhizobacteria (PGPR) initially isolated from the rhizosphere of pristine grassland in South Africa, and its ability to enhance growth was previously evaluated on maize (Zea mays L.). Here, the bacterium was tested with the aim of investigating its role in improving the nodulation and growth of the forage legume lucerne (Medicago sativa L.) when it is co-inoculated with the rhizobial symbionts of this legume in the glasshouse. When the co-inoculation resulted in a statistically significant (P = 0·05) increase in the number of nodules and improved plant biomass compared with single inoculation, we sequenced and analysed its genome to gain a better understanding of the genetic determinants responsible for the observed PGPR traits. The Illumina HiSeq 2500-sequenced genome resulted in 92 scaffolds, with an N50 of 322 407 bp, a total draft genome size of 7 788 045 bp and GC content of 66·2%. Analysis of the genome sequence confirmed the presence of a number of essential genes that code for various PGPR traits. The main plant beneficial genes associated with PGPR traits in Burkholderia sp. Nafp2/4-1b include pyoverdine siderophores biosynthesis gene (PvdF); acdS that codes for 1-aminocyclopropane-1-carboxylate (ACC) deaminase; the tryptophan synthase genes involved in auxin biosynthesis (TSA1, TSB1) and the pqqABCDE operon related to phosphate solubilization. This study generated valuable information on the potential of the PGPR Burkholderia sp. strain Nafp2/4-1b as an effective commercial inoculant, which warrants further formulation and field application studies before developing it into a low cost, environmentally safe and effective biofertilizer.

中文翻译：

阐明伯克霍尔德菌的关键植物生长促进（PGPR）性状。Nafp2/4-1b (=SARCC-3049) 使用无菌检测和全基因组序列分析

伯克霍尔德菌属 Nafp2/4-1b (=SARCC-3049) 是一种植物生长促进根际细菌 (PGPR)，最初从南非原始草原的根际分离出来，之前在玉米 ( Zea mays L.)上对其促进生长的能力进行了评估。 . 在这里，对该细菌进行了测试，目的是研究其在温室中与这种豆科植物的根瘤菌共生体共同接种时，其在改善草料豆科植物苜蓿 ( Medicago sativa L.)的结瘤和生长方面的作用。当共同接种导致统计学显着（P = 0·05) 与单次接种相比，根瘤数量增加并改善植物生物量，我们对其基因组进行了测序和分析，以更好地了解导致观察到的 PGPR 性状的遗传决定因素。Illumina HiSeq 2500测序的基因组得到92个支架，N50为322 407 bp，基因组草图总大小为7 788 045 bp，GC含量为66·2%。对基因组序列的分析证实了许多编码各种 PGPR 性状的必需基因的存在。Burkholderia sp.中与 PGPR 性状相关的主要植物有益基因。Nafp2/4-1b 包括pyoverdine 铁载体生物合成基因（PvdF）；编码为 1 的acdS -氨基环丙烷-1-羧酸（ACC）脱氨酶；涉及生长素生物合成的色氨酸合酶基因（TSA1、TSB1）和与磷酸盐溶解相关的pqqABCDE操纵子。这项研究产生了关于 PGPR Burkholderia sp.潜力的宝贵信息。菌株 Nafp2/4-1b 作为一种有效的商业接种剂，在将其开发成低成本、环境安全和有效的生物肥料之前，需要进一步的配方和现场应用研究。

更新日期：2021-10-28

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