Abstract

Nanoparticles (NPs) are known as fundamental building blocks of nanotechnology. In this work, silver nanoparticles (AgNPs) were synthesized using Stachys annua L. sabsp. annua var. annua. The structure of AgNPs was elucidated by FTIR, UV-Vis, XRD, SEM analyses. The UV-Vis spectrum revealed the maximum absorption at 469 nm and SEM spectral analysis showed the formation of spherical shape AgNPs with an average size of 75.21 nm. XRD analysis at 2θ degrees of 38.1°, 44.3°, 64.4°, and 77.4° can be assigned to the (111), (200), (220) and (311) face-centered cubic crystalline structure. Antioxidant activity of extract and AgNPs was executed using the DPPH^• scavenging, ABTS^•+ scavenging, and reducing power assays. AgNPs and extract revealed the higher DPPH^• free radical scavenging effect with the values of 10.2 ± 0.34 (lC₅₀, µg/mL) and 9.26 ± 0.37 (lC₅₀, µg/mL) than the standard BHT (11.13 ± 0.10, lC₅₀, µg/mL). ABTS^•+ activity of AgNPs (4.11 ± 0.04, lC₅₀, µg/mL) and extract (3.96 ± 0.10) were found higher than that of the standard BHT. In reducing power assay, the activity of AgNPs (2.61 ± 0.02, µmol TE/mg extract) and extract (3.05 ± 0.15, µmol TE/mg extract) was lower than BHT. Hence, extract and AgNPs could be promising materials for antioxidant agents.

摘要

纳米粒子 (NPs) 被称为纳米技术的基本组成部分。在这项工作中，银纳米粒子 (AgNPs) 使用Stachys annua L. sabsp 合成。年鉴变种 青蒿 通过FTIR、UV-Vis、XRD、SEM分析阐明了AgNPs的结构。UV-Vis 光谱显示在 469 nm 处有最大吸收，SEM 光谱分析显示形成了平均尺寸为 75.21 nm 的球形 AgNPs。在 38.1°、44.3°、64.4° 和 77.4° 的2 θ 度下的XRD 分析可归属于 (111)、(200)、(220) 和 (311) 面心立方晶体结构。使用 DPPH ^•清除剂、ABTS ^{•+执行提取物和 AgNPs 的抗氧化活性}清除和降低功率测定。AgNPs 和提取物显示出比标准 BHT (11.13 ± 0.10, lC _{50 ) 更高的 DPPH} ^•自由基清除效果，其值为 10.2 ± 0.34 (LC ₅₀ , µg/mL) 和 9.26 ± 0.37 (LC ₅₀ , µg/mL) ，微克/毫升）。AgNPs (4.11 ± 0.04, lC ₅₀ , µg/mL) 和提取物 (3.96 ± 0.10) 的ABTS ^•+活性高于标准 BHT。在还原能力测定中，AgNPs (2.61 ± 0.02, µmol TE/mg 提取物) 和提取物 (3.05 ± 0.15, µmol TE/mg 提取物) 的活性低于 BHT。因此，提取物和 AgNPs 可能是有前途的抗氧化剂材料。