Intracellular density is a novel indicator of differentiation stages of murine osteoblast lineage cells,Journal of Cellular Biochemistry

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Intracellular density is a novel indicator of differentiation stages of murine osteoblast lineage cells
Journal of Cellular Biochemistry ( IF 3.0 ) Pub Date : 2021-08-24 , DOI: 10.1002/jcb.30135
Yuki Itoh ₁ , Shousaku Itoh ₁ , Haruna Naruse ₁ , Takumi Kagioka ₁ , Mai Thi Hue ₁ , Makoto Abe ₂ , Mikako Hayashi ₁

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Osteoblasts are primary bone-making cells originating from mesenchymal stem cells (MSCs) in the bone marrow. The differentiation of MSCs to mature osteoblasts involves an intermediate stage called preosteoblasts, but the details of this process remain unclear. This study focused on the intracellular density of immature osteoblast lineage cells and hypothesized that the density might vary during differentiation and might be associated with the differentiation stages of osteoblast lineage cells. This study aimed to clarify the relationship between intracellular density and differentiation stages using density gradient centrifugation. Primary murine bone marrow stromal cell cultures were prepared in an osteogenic induction medium, and cells were separated into three fractions (low, intermediate, and high-density). The high-density fraction showed elevated expression of osteoblast differentiation markers (Sp7, Col1a1, Spp1, and Bglap) and low expression of MSC surface markers (Sca-1, CD73, CD105, and CD106). In contrast, the low-density fraction showed a high expression of MSC surface markers. These results indicated that intracellular density increased during differentiation from preosteoblasts to committed osteoblasts. Intracellular density may be a novel indicator for osteoblast differentiation stages. Density gradient centrifugation is a novel technique to study the process by which preosteoblasts transform into bone-forming cells.

中文翻译：

细胞内密度是小鼠成骨细胞谱系细胞分化阶段的新指标

成骨细胞是起源于骨髓间充质干细胞 (MSCs) 的主要造骨细胞。MSCs 向成熟成骨细胞的分化涉及一个称为前成骨细胞的中间阶段，但该过程的细节仍不清楚。本研究关注未成熟成骨细胞谱系细胞的细胞内密度，并假设该密度可能在分化过程中发生变化，并可能与成骨细胞谱系细胞的分化阶段有关。本研究旨在使用密度梯度离心阐明细胞内密度和分化阶段之间的关系。在成骨诱导培养基中制备原代鼠骨髓基质细胞培养物，并将细胞分成三个部分（低、中和高密度）。Sp7、Col1a1、Spp1和Bglap）和 MSC 表面标志物（Sca-1、CD73、CD105 和 CD106）的低表达。相比之下，低密度部分表现出 MSC 表面标志物的高表达。这些结果表明，在从前成骨细胞分化为定型成骨细胞的过程中，细胞内密度增加。细胞内密度可能是成骨细胞分化阶段的新指标。密度梯度离心是一种研究前成骨细胞转化为骨形成细胞过程的新技术。

更新日期：2021-08-24

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