The population of Hynobius yiwuensis has decreased dramatically due to urbanization and human activities. In order to better monitor and protect this species, 36 single nucleotide polymorphism (SNP) markers were developed using double digest restriction-site associated DNA sequencing (ddRAD-seq). The frequency of minor allele per locus ranged from 0.0132 to 0.3594, while the observed and expected heterozygosity varied from 0.0263 to 0.5278 (average 0.1565) and from 0.0260 to 0.4604 (average 0.1396), respectively. The inbreeding coefficient ranged from − 0.2643 to 0.0000. No loci showed significant departure from Hardy–Weinberg equilibrium (P_HWE > 0.05). These polymorphic SNP markers will be useful for the study of conservation genetics in H. yiwuensis.

由于城市化和人类活动，义乌鹬的数量急剧减少。为了更好地监测和保护该物种，使用双酶切限制位点相关 DNA 测序 (ddRAD-seq) 开发了 36 个单核苷酸多态性 (SNP) 标记。每个位点的次要等位基因频率范围为 0.0132 到 0.3594，而观察到的和预期的杂合度分别从 0.0263 到 0.5278（平均 0.1565）和从 0.0260 到 0.4604（平均 0.1396）不等。近交系数的范围为 - 0.2643 至 0.0000。没有位点显示出显着偏离 Hardy-Weinberg 平衡（P _HWE > 0.05）。这些多态性 SNP 标记将有助于义乌血吸虫的保护遗传学研究.