Bioethanol is considered one of the most promising next-generation automotive fuels, as it is carbon neutral and can be produced from renewable resources, like lignocellulosic materials. The present research investigation aimed to utilize the elephant ear plant, a hazardous plant (weed) also considered an invasive species, as a font of non-edible lignocellulosic biomass for bioethanol production. The freshly collected elephant ear plant (leaves and stalk) was chopped into small pieces (1–2 cm) and then homogenized to a paste using a mechanical grinder. The sample pretreatment was done by flying ash for three different time durations (T1 = 0 min, T2 = 15 min, and T3 = 30 min) with 3 replications. All treatment samples were measured for total sugar and reducing sugar content. The concentration of reducing sugar archived was T1 = 0.771 ± 0.1 mg/mL, T2 = 0.907 ± 0.032 mg/mL, and T3 = 0.895 ± 0.039 mg/mL, respectively. The results revealed that the chemical composition was different among treatments. The hydrolysis was performed using cellulase enzymes at 35 °C for the hydrolysis process. The hydrolysate was inoculated with 1% of S. cerevisiae and maintained at room temperature without oxygen for 120 h. Bioethanol concentration was measured by using an ebulliometer. The efficient ethanol percentage was 1.052 ± 0.03 mg/mL achieved after the fermentation. Therefore, the elephant ear plant invasive weed could be an efficient feedstock plant for future bioethanol production.

生物乙醇被认为是最有前途的下一代汽车燃料之一，因为它是碳中性的，并且可以由木质纤维素材料等可再生资源生产。本研究旨在利用象耳植物，一种危险植物（杂草），也被认为是一种入侵物种，作为一种用于生物乙醇生产的非食用木质纤维素生物质。将新鲜收集的象耳植物（叶和茎）切成小块（1-2 厘米），然后使用机械研磨机均质成糊状。样品预处理通过飞灰进行三个不同的持续时间（T 1 = 0 min、T 2 = 15 min 和T3 = 30 分钟），重复 3 次。测量所有处理样品的总糖和还原糖含量。存档的还原糖浓度分别为T 1 = 0.771 ± 0.1 mg/mL、T 2 = 0.907 ± 0.032 mg/mL 和T 3 = 0.895 ± 0.039 mg/mL。结果表明，不同处理的化学成分不同。在水解过程中使用纤维素酶在 35°C 下进行水解。用 1% 的S. cerevisiae接种水解产物并在室温无氧条件下保持 120 h。使用沸腾计测量生物乙醇浓度。发酵后达到的有效乙醇百分比为 1.052 ± 0.03 mg/mL。因此，象耳植物入侵杂草可能是未来生物乙醇生产的有效原料植物。