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CRISPR-PN2: a flexible and genome-aware platform for diverse CRISPR experiments in parasitic nematodes.
Biotechniques ( IF 2.2 ) Pub Date : 2021-08-23 , DOI: 10.2144/btn-2021-0056
Damien M O'Halloran 1
Affiliation  

Parasitic nematodes represent a significant threat to human health, causing diseases of major socioeconomic importance worldwide. Central to controlling infections of parasitic nematodes is a more detailed molecular picture of host specificity, parasite activation and immune suppression. CRISPR technology holds huge potential for researchers in the field of parasitic nematology, as it provides a powerful genetic tool to dissect questions in parasite biology. To expedite the development of CRISPR technology in parasitic nematodes, software is required to facilitate the design of effective and specific sgRNA sequences. Here, the author introduces CRISPR-PN2, a comprehensive web-based platform that provides flexible use control over the automated design of specific gRNA sequences for CRISPR experiments in parasitic nematodes.

中文翻译:


CRISPR-PN2:一个灵活的基因组感知平台,用于寄生线虫的多种 CRISPR 实验。



寄生线虫对人类健康构成重大威胁,在全世界引起具有重大社会经济意义的疾病。控制寄生线虫感染的核心是宿主特异性、寄生虫激活和免疫抑制的更详细的分子图景。 CRISPR 技术对于寄生线虫学领域的研究人员来说具有巨大的潜力,因为它提供了一种强大的遗传工具来剖析寄生虫生物学中的问题。为了加快寄生线虫中 CRISPR 技术的发展,需要软件来促进有效且特异的 sgRNA 序列的设计。在此,作者介绍了 CRISPR-PN2,这是一个基于网络的综合平台,可为寄生线虫 CRISPR 实验的特定 gRNA 序列的自动化设计提供灵活的使用控制。
更新日期:2021-08-23
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