Thanks to its ability to isolate previously uncultured bacterial species, culturomics has dynamized the study of the human microbiota. A new bacterial species, Gemella massiliensis Marseille-P3249^T, was isolated from a sputum sample of a healthy French man. Strain Marseille-P3249^T is a facultative anaerobe, catalase-negative, Gram positive, coccus, and unable to sporulate. The major fatty acids were C_16:0 (34%), C_18:1n9 (28%), C_18:0 (15%) and C_18:2n6 (13%). Its 16S rRNA sequence exhibits a 98.3% sequence similarity with Gemella bergeri strain 617-93^T, its phylogenetically closest species with standing in nomenclature. Its digital DNA–DNA hybridization (dDDH) and OrthoANI values with G. bergeri of only 59.7 ± 5.6% and 94.8%, respectively. These values are lower than the thresholds for species delineation (> 70% and > 95%, respectively). This strain grows optimally at 37 °C and its genome is 1.80 Mbp long with a 30.5 mol% G + C content. Based on these results, we propose the creation of the new species Gemella massilienis sp. nov., strain Marseille-P3249^T (= CSUR P3249 = DSMZ 103940).

由于能够分离以前未培养的细菌物种，培养组学使人类微生物群的研究充满活力。从一名健康的法国男子的痰液样本中分离出一种新细菌Gemella massiliensis Marseille-P3249 ^T。菌株 Marseille-P3249 ^T是一种兼性厌氧菌，过氧化氢酶阴性，革兰氏阳性，球菌，不能形成孢子。主要脂肪酸为 C _16:0 (34%)、C _18:1n9 (28%)、C _18:0 (15%) 和 C _18:2n6 (13%)。其 16S rRNA 序列与Gemella bergeri菌株 617-93 ^T具有 98.3% 的序列相似性, 它在系统发育上最接近的物种，在命名法中具有地位。其与G. bergeri的数字 DNA-DNA 杂交 (dDDH) 和 OrthoANI 值分别仅为 59.7 ± 5.6% 和 94.8%。这些值低于物种划分的阈值（分别 > 70% 和 > 95%）。该菌株在 37 °C 下生长最佳，其基因组长 1.80 Mbp，G + C 含量为 30.5 mol%。基于这些结果，我们建议创建新物种Gemella massilienis sp。11 月，菌株 Marseille-P3249 ^T（= CSUR P3249 = DSMZ 103940）。