Spindle and kinetochore-associated complex subunit 3 (SKA3) is reportedly a key contributor to the progression of various cancers. The present work aimed to evaluate the possible role of SKA3 in cutaneous melanoma (CM). A high SKA3 level was found in CM tissues and predicted a poor prognosis. SKA3 silencing markedly repressed the proliferation, invasion, and epithelial-mesenchymal transition and induced the apoptosis of CM cells. SKA3 silencing decreased the phosphorylation of PI3K and Akt. Akt inhibition markedly reversed SKA3 overexpression-induced oncogenic effects on CM cells. SKA3 silencing significantly prohibited the formation and growth of CM-derived xenograft tumors in nude mice in vivo. Our findings demonstrated SKA3 inhibition repressed the progression of CM by downregulating the PI3K/Akt pathway. This study indicates that SKA3 has potential as an anticancer candidate for CM.

中文翻译：

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抑制纺锤体和动粒相关复合亚基3通过影响PI3K/Akt通路抑制皮肤黑色素瘤的增殖和侵袭并诱导细胞凋亡

据报道，纺锤体和动粒相关的复合亚基 3 (SKA3) 是各种癌症进展的关键因素。目前的工作旨在评估 SKA3 在皮肤黑色素瘤 (CM) 中的可能作用。在 CM 组织中发现高 SKA3 水平并预测预后不良。SKA3沉默显着抑制了CM细胞的增殖、侵袭和上皮-间质转化，并诱导了CM细胞的凋亡。SKA3 沉默降低了 PI3K 和 Akt 的磷酸化。Akt 抑制显着逆转了 SKA3 过表达诱导的对 CM 细胞的致癌作用。SKA3 沉默显着抑制了裸鼠体内 CM 衍生的异种移植肿瘤的形成和生长。我们的研究结果表明，SKA3 抑制通过下调 PI3K/Akt 通路来抑制 CM 的进展。