In this paper, a novel silicon-based light-addressable potentiometric sensor (LAPS) has been designed for the detection of 1,5-anhydroglucitol (1,5-AG) in human serum. Reduced graphene oxide-chitosan-ferrocene (RGO-CS-Fc)/AuNPs nanohybrids and pyranose oxidase (PROD) enzyme is used to fabricate biological sensitive membrane unit by layer-by-layer assembly technology. When a bias voltage is provided to the LAPS system, the catalytic oxidation reaction between 1,5-AG and PROD to produce H₂O₂. The by-product H₂O₂ can oxidize Fc(Fe²⁺) ions in RGO-CS-Fc nanohybrids into Fc(Fe³⁺) ions, which cause the potential of the sensitive membrane surface to change and the potential shift of I-V curve will generate a corresponding offset response. Under the optimal conditions, the potential shift of the LAPS is linearly related to the concentration of 1,5-AG at 10 µg·mL⁻¹ –350 µg·mL⁻¹ with the correlation coefficient of 0.97414. The sensitivity is 0.44273 mV/µg·mL⁻¹ and the lowest detection limit is 10 µg·mL⁻¹. In addition, the biosensor showed good specificity, acceptable stability and satisfactory recovery rates (91.28%-107.66%), which would be a potential testing methods in actual clinical samples.

在本文中，设计了一种新型硅基光寻址电位传感器 (LAPS)，用于检测人血清中的 1,5-脱水葡萄糖醇 (1,5-AG)。还原氧化石墨烯-壳聚糖-二茂铁(RGO-CS-Fc)/AuNPs纳米杂化物和吡喃糖氧化酶(PROD)通过逐层组装技术制备生物敏感膜单元。当向 LAPS 系统提供偏置电压时，1,5-AG 和 PROD 之间的催化氧化反应生成 H ₂ O ₂。副产物 H ₂ O ₂可以将RGO-CS-Fc 纳米杂化物中的Fc(Fe ^{2+ ) 离子氧化成 Fc(Fe 3+}) 离子，引起敏感膜表面电位发生变化，IV 曲线的电位偏移会产生相应的偏移响应。在最佳条件下，LAPS的电位偏移与10 µg·mL ^-1 –350 µg·mL ^-1的1,5-AG浓度呈线性相关，相关系数为0.97414。灵敏度为0.44273 mV/µg·mL ^-1，最低检测限为10 µg·mL ^-1。此外，该生物传感器具有良好的特异性、可接受的稳定性和令人满意的回收率（91.28%-107.66%），这将是一种潜在的临床样品检测方法。