Phase microscopy is widely used to image unstained biological samples. However, most phase imaging techniques require transmission geometries, making them unsuited for thick sample applications. Moreover, when applied to volumetric imaging, phase imaging generally requires large numbers of measurements, often making it too slow to capture live biological processes with fast 3D index-of-refraction variations. By combining oblique back-illumination microscopy and a z-splitter prism, we perform phase imaging that is both epi-mode and multifocus, enabling high-speed 3D phase imaging in thick, scattering tissues with a single camera. We demonstrate here 3D qualitative phase imaging of blood flow in chick embryos over a field of view of 546 × 546 × 137 µm³ at speeds up to 47 Hz.

中文翻译：

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厚组织中的高速多焦点相位成像


相差显微镜广泛用于对未染色的生物样品进行成像。然而，大多数相位成像技术需要透射几何形状，这使得它们不适合厚样品应用。此外，当应用于体积成像时，相位成像通常需要大量测量，这通常使得捕获具有快速 3D 折射率变化的活体生物过程变得太慢。通过结合倾斜背照式显微镜和 z 分光棱镜，我们可以执行外延模式和多焦点相位成像，从而能够使用单个相机在厚的散射组织中进行高速 3D 相位成像。我们在此展示了鸡胚胎血流的 3D 定性相位成像，视野范围为 546 × 546 × 137 µm ³ ，速度高达 47 Hz。