Development of Poloxamer Hydrogels Containing Antibacterial Guanidine-Based Polymers for Healing of Full-Thickness Skin Wound,ACS Biomaterials Science & Engineering

当前位置： X-MOL 学术 › ACS Biomater. Sci. Eng. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Development of Poloxamer Hydrogels Containing Antibacterial Guanidine-Based Polymers for Healing of Full-Thickness Skin Wound
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2021-08-23 , DOI: 10.1021/acsbiomaterials.1c00600
Peipei Feng ₁ , Haofeng Qiu ₁ , Yang Luo ₁ , Jiaying Hu ₁ , Yuhao Cao ₁ , Qian Pang ₁ , Xianbo Mou ₁ , Ruixia Hou ₁ , Wenjia Hou ₁ , Yabin Zhu ₁

Affiliation

A series of hydrogels containing guanidine-based polymers using a poloxamer as the matrix were prepared to provide novel wound dressings with antibacterial and repairing-promotion properties for skin wounds. Herein, we developed a series of antibacterial hydrogels, the cationic guanidine-based polymer polyhexamethylene guanidine hydrochloride (PHMG) with poloxamer aqueous solution (12%, w/w) simplified as PHMGP, chitosan (CS)-cross-linked PHMG (referred to as PHMC) with poloxamer aqueous solution simplified as PHMCP, and hyaluronic acid (HA)-modified PHMG (referred to as PHMH) with poloxamer aqueous solution simplified as PHMHP, for enhancing full-thickness skin wound healing. The characterizations, antimicrobial activity, cytotoxicity, and in vivo full-thickness wound-healing capability of these hydrogels were analyzed and evaluated. The results show that though PHMGP possesses great bactericide properties, its cytotoxicity is too strong to support skin regeneration. However, after modified with CS or HA, PHMCP and PHMHP showed good biocompatibility and antimicrobial properties against Gram-positive and Gram-negative bacteria that are commonly present in injured skin. Both PHMCP and PHMHP hydrogels exhibited upgraded wound-healing efficiency in full-thickness skin defects, characterized by a shorter wound closure time, faster re-regeneration, and the earlier formation of skin appendages, compared with those of control or pure poloxamer treatments. Their biological mechanism was detected. Both PHMCP and PHMHP can regulate the related biofactors during the skin repair process such as interleukin-1β (IL-1β), interleukin-6 (IL-6), transforming growth factor beta-1(TGF-β1), alpha-smooth muscle actin (α-SMA), and vascular endothelial growth factor, to promote wound healing with less serious scarring. In short, hydrogels with excellent capabilities to inhibit microorganism infection and promote wound healing were developed, which will shed light on designing and producing wound dressings with promising applications in future.

中文翻译：

含有抗菌胍基聚合物的泊洛沙姆水凝胶用于全层皮肤伤口愈合的开发

以泊洛沙姆为基质制备了一系列含有胍基聚合物的水凝胶，为皮肤伤口提供具有抗菌和修复促进特性的新型伤口敷料。在此，我们开发了一系列抗菌水凝胶，阳离子胍基聚合物聚六亚甲基胍盐酸盐 (PHMG) 与泊洛沙姆水溶液 (12%, w/w) 简化为 PHMGP、壳聚糖 (CS) 交联 PHMG（简称如PHMC）与泊洛沙姆水溶液简化为PHMCP，以及透明质酸（HA）修饰的PHMG（简称PHMH）与泊洛沙姆水溶液简化为PHHMHP，用于促进全层皮肤伤口愈合。分析和评估了这些水凝胶的特性、抗菌活性、细胞毒性和体内全层伤口愈合能力。结果表明，虽然PHMGP具有很强的杀菌性能，但其细胞毒性太强，无法支持皮肤再生。然而，经 CS 或 HA 修饰后，PHMCP 和 PHMHP 对损伤皮肤中常见的革兰氏阳性菌和革兰氏阴性菌显示出良好的生物相容性和抗菌性能。与对照或纯泊洛沙姆治疗相比，PHMCP 和 PHMHP 水凝胶在全层皮肤缺损中表现出更高的伤口愈合效率，其特点是伤口闭合时间更短、再生更快、皮肤附属物形成更早。检测到它们的生物学机制。PHMCP和PHHMHP均可调节皮肤修复过程中的相关生物因子，如白细胞介素1β（IL-1β）、白细胞介素6（IL-6）、转化生长因子β-1（TGF-β1）、α-平滑肌肌动蛋白 (α-SMA) 和血管内皮生长因子，以促进伤口愈合，疤痕较轻。简而言之，开发了具有优异抑制微生物感染和促进伤口愈合能力的水凝胶，这将为设计和生产具有广阔应用前景的伤口敷料提供启示。

更新日期：2021-09-13

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11