Antibacterial Activity and Synergetic Mechanism of Carbon Dots against Gram-Positive and -Negative Bacteria,ACS Applied Bio Materials

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Antibacterial Activity and Synergetic Mechanism of Carbon Dots against Gram-Positive and -Negative Bacteria
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2021-08-23 , DOI: 10.1021/acsabm.1c00618
Jiarong Liang ₁ , Wei Li _{1,

2} , Jianying Chen ₁ , Xiaoman Huang ₁ , Yingliang Liu _{1,

2} , Xuejie Zhang _{1,

2} , Wei Shu ₃ , Bingfu Lei _{1,

2} , Haoran Zhang ₁

Affiliation

Carbon dots (CDs) with exciting photoluminescence characteristics, mild toxicity, and good biocompatibility are the research hotspots in biomedical application. Here, a compact antibacterial activity of CDs from levofloxacin hydrochloride is reported. The obtained CDs with an average size of 1.27 nm have fascinating antibacterial properties against both gram-positive and negative bacteria, with minimum inhibitory concentrations (MICs) of 64, 128, 64, and 128 μg/mL for Escherichia coli (E. coli),Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus), and Bacillus subtilis (B. subtilis). The antibacterial processes of CDs from extracellular to intracellular were demonstrated, including physical/chemical binding to membrane, wrapping on the surface, destruction of the cell membrane, and promoting reactive oxygen species (ROS) production into the cell without additional light or oxidant. Surprisingly, CDs exert moderate cytotoxicity on mammalian cells at the equivalent bactericidal concentration, in which the cell viability is more than 80% at 100 μg/mL of CDs. The investigation of antibacterial CDs may provide a useful avenue for further exploiting CD-based nano-bactericides in biomedical applications.

中文翻译：

碳点对革兰氏阳性和阴性菌的抗菌活性及协同作用机制

具有令人兴奋的光致发光特性、毒性小、生物相容性好的碳点（CDs）是生物医学应用的研究热点。在这里，报道了盐酸左氧氟沙星的 CD 具有紧凑的抗菌活性。获得的平均尺寸为 1.27 nm 的 CD 对革兰氏阳性和阴性细菌均具有出色的抗菌特性，对大肠杆菌( E. coli )的最低抑菌浓度 (MIC) 分别为 64、128、64 和 128 μg/mL 、铜绿假单胞菌( P. aeruginosa )、金黄色葡萄球菌( S. aureus ) 和枯草芽孢杆菌( B. subtilis )）。证明了 CDs 从细胞外到细胞内的抗菌过程，包括物理/化学结合膜、包裹在表面、破坏细胞膜以及在没有额外光或氧化剂的情况下促进活性氧 (ROS) 进入细胞。令人惊讶的是，CDs 在同等杀菌浓度下对哺乳动物细胞具有中等的细胞毒性，其中在 100 μg/mL 的 CDs 下，细胞活力超过 80%。抗菌 CD 的研究可能为在生物医学应用中进一步开发基于 CD 的纳米杀菌剂提供有用的途径。

更新日期：2021-09-20

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