Macropanax dispermus is traditionally used to treat various diseases by ethnic people. The present research reports the pharmacological properties with phytochemical profiling of the crude extracts of M. dispermus leaves (MDML), its n-hexane (MDHL), carbon tetrachloride (MDTL), chloroform (MDCL), ethyl acetate (MDEL), and aqueous (MDAL) fractions, and crude methanol extracts of its stem barks (MDMS). The in vitro thrombolytic activity was done on human erythrocytes whereas the cytotoxic activity was done by brine shrimp lethality assay. The in vivo analgesic activity was examined by acetic acid-induced writhing, tail immersion, and formalin-induced paw licking method. In contrast, antipyretic activity was done by the brewer’s yeast-induced pyrexia method. MDHL and MDMS showed 37.05% and 42.21% of significant (p < 0.01) thrombolytic activity, respectively. MDCL and MDMS showed the lower LC50 values of 23.15 and 37.11 µg/ml during cytotoxicity test, respectively. In acetic acid writhing method, MDTL and MDEL showed significant (p < 0.001) inhibition of writhing by 79.34% and 80.17%, respectively. MDMS showed significant (p < 0.001) maximal possible effect (%MPE) of 45.95%, 62.26%, 65.79%, 89.69% and elongation of time in pain reaction of 48.53%, 60.28%, 58.76%, and 70.14% at 30, 60, 90, and 120 min intervals, respectively. MDML at 400 mg/kg exhibited significant (p < 0.001) 82.72% of inhibition of pain at the late phases. MDEL at 400 mg/kg of dose exhibited significant (p < 0.001) reduction of rectal temperature by 36.31%, 62.42%, 89.81%,, and 96.82% at 1, 2, 3, and 4 h intervals, respectively. The current research suggests that the plant extracts possess potential thrombolytic, cytotoxic, analgesic, and antipyretic activities.

中文翻译：

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大人参（五加科）叶和茎皮不同提取物的植物化学和药理研究：一种有前途的民族药用植物

Macropanax dispermus 传统上被少数民族用来治疗各种疾病。本研究报告了松柏叶粗提取物 (MDML)、正己烷 (MDHL)、四氯化碳 (MDTL)、氯仿 (MDCL)、乙酸乙酯 (MDEL) 和水溶液的药理特性。 (MDAL) 馏分，以及其茎皮 (MDMS) 的粗甲醇提取物。体外溶栓活性是对人红细胞进行的，而细胞毒性活性是通过盐水虾致死率测定进行的。通过乙酸诱导的扭体、尾部浸泡和福尔马林诱导的舔爪法来检查体内镇痛活性。相比之下，解热活性是通过啤酒酵母诱导的发热方法完成的。MDHL 和 MDMS 分别显示 37.05% 和 42.21% 的显着（p < 0.01）溶栓活性，分别。MDCL 和 MDMS 在细胞毒性试验中分别显示出较低的 LC50 值，分别为 23.15 和 37.11 µg/ml。在醋酸扭体法中，MDTL 和 MDEL 显示出显着的（p < 0.001）扭体抑制率分别为 79.34% 和 80.17%。MDMS 显示显着 (p < 0.001) 最大可能效应 (%MPE) 为 45.95%、62.26%、65.79%、89.69%，疼痛反应时间延长为 48.53%、60.28%、58.76% 和 7301%。分别为 60、90 和 120 分钟的间隔。400 mg/kg 的 MDML 在晚期表现出显着的 (p < 0.001) 82.72% 的疼痛抑制。400 mg/kg 剂量的 MDEL 表现出显着 (p < 0.001) 直肠温度降低 36.31%、62.42%、89.81% 和 96.82%，间隔时间为 1、2、3 和 4 小时。