Proteins and enzymes with potential therapeutic function have been widely used in drug delivery. Meanwhile, nanocrystals (NCs) have showed their advantage as in vivo fluorescent indicators. Combining of these two kinds of materials can result in multifunctional composites. Here, a one-step synthesis of protein–NCs nanocomposites with near infrared (NIR) fluorescence and efficient therapeutic function was developed. Ag NCs were formed using ribonucleases A (RNase A) enzyme as a stabilizer. Meanwhile, the RNase A with long chain rapped around Ag NCs and formed RNase A–Ag nanocomposites. The nanocomposites remained both the NIR fluorescence of Ag NCs and antitumor activity of RNase A. The inhibition of the growth of tumor cells by the nanocomposites was confirmed. This study developed an efficient drug delivery system through combination of the protein and nanoparticles, which had potential clinical application in the future.

中文翻译：

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一步合成核糖核酸酶 A-Ag 纳米复合材料作为用于体内递送的荧光纳米药物

具有潜在治疗功能的蛋白质和酶已广泛用于药物递送。同时，纳米晶体（NCs）已显示出其优势：体内荧光指示剂。这两种材料的结合可以产生多功能复合材料。在这里，开发了一种具有近红外 (NIR) 荧光和有效治疗功能的蛋白质-NCs 纳米复合材料的一步合成方法。使用核糖核酸酶 A（RNase A）酶作为稳定剂形成 Ag NC。同时，长链的 RNase A 在 Ag NCs 周围敲击并形成 RNase A-Ag 纳米复合材料。纳米复合材料保持了Ag NCs的NIR荧光和RNase A的抗肿瘤活性。证实了纳米复合材料对肿瘤细胞生长的抑制作用。本研究通过蛋白质和纳米粒子的结合开发了一种高效的药物递送系统，在未来具有潜在的临床应用价值。