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Study on the Composition and Physiological Activity of the Essential Oils and Extracts of Cinnamomum camphora Fruit
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2021-08-22 , DOI: 10.1002/cbdv.202100201
Chenyuan Lv 1 , Linyu Hao 1 , Xinang Cui 1 , Fengping Yi 1 , Chang Su 1
Affiliation  

Supercritical carbon dioxide (SC-CO2), hydrodistillation (HDO), ethanol extraction (EE), and petroleum ether extraction (PE) were used to extract the essential oil and extracts of Cinnamomum camphora fruit in this study. Gas chromatography-mass spectrometry was used to identify the volatile components of essential oils and extracts, and 63 compounds were identified. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay and Ferric reducing ability of plasma (FRAP) assays and the inhibition experiment of bacteria and fungi (Staphylococcus aureus (S. aureus), Hay bacillus (H. bacillus), Escherichia coli (E. coli), Aspergillus niger (A. niger), Candida albicans (C. albicans)) showed these essential oils and extracts indicated antioxidant and antibacterial activities. S. aureus was the most sensitive to the essential oil (MIC=0.08 mg/ml). Combined with the Brine Shrimp Lethality Test (BSLT) experiment, HDO (LD50=68.21 μg/ml) was considered to have the most potential natural preservative. Subsequently, the inhibitory mechanism of HDO on bacteria and fungi was explored through extracellular conductivity and SEM, and the possibility of HDO to preserve the freshness of bananas was verified through banana shelf-life experiments. The results suggested these essential oils and extracts of Cinnamomum camphora fruit indicated effectively inhibit the growth of microorganisms on the surface of bananas, extend the shelf-life, and have the potential to become a natural antiseptic ingredient.

中文翻译:

香樟精油及提取物的组成及生理活性研究

本研究采用超临界二氧化碳 (SC-CO 2 )、加氢蒸馏 (HDO)、乙醇萃取 (EE) 和石油醚萃取 (PE) 萃取香樟果实的精油和提取物。采用气相色谱-质谱法鉴定精油和提取物的挥发性成分,鉴定出 63 种化合物。2,2-Diphenyl-1-picrylhydrazyl (DPPH) 和 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) 自由基清除测定和血浆铁还原能力 (FRAP) 测定和抑制实验细菌和真菌(金黄色葡萄球菌S. aureus)、干草杆菌H. bacillus)、大肠杆菌E. coli)、黑曲霉A. niger)、白色念珠菌C. albicans))显示这些精油和提取物具有抗氧化和抗菌活性。金黄色葡萄球菌对精油最敏感(MIC=0.08 mg/ml)。结合盐水虾杀伤力测试 (BSLT) 实验,HDO (LD 50=68.21 μg/ml)被认为是最有潜力的天然防腐剂。随后,通过细胞外电导和扫描电镜研究了HDO对细菌和真菌的抑制机制,并通过香蕉保质期实验验证了HDO对香蕉保鲜的可能性。结果表明,这些香樟精油和香樟果实提取物能有效抑制香蕉表面微生物的生长,延长保质期,具有成为天然防腐成分的潜力。
更新日期:2021-08-22
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