Plants modify their carbon allocation as a response to low water availability. The objective of this study was to evaluate, using a ¹⁴CO₂ pulse-chase analysis, the effect of moisture restriction on biomass production in common bean plants var. OTI. The plants were maintained with irrigation until the beginning of pods filling; then, three groups were formed, kept at 100%, 75% or 50% field capacity (FC). After 10 days, ¹⁴CO₂ gas was supplied to the plants for 4 hr. The plants were harvested at 1, 3 and 7 days after applying the label. Ripe fruits imported more than 50% of the total ¹⁴C. Particularly, the label presented greater changes in pericarps of stage III of pod development. The fructose concentration doubled that of the glucose and decreased with the pod age; sucrose concentration increased in pericarps in stages III and IV of pod development in relation to those in stage II. The sucrose decrease only in stage II pericarps on day 7, as well as the starch concentration that decreased by half in the 50% FC condition. The latter coincided with the highest amylolytic activity as evaluated in native gels. These findings open new opportunities to research the carbon allocation mechanism under moisture restriction.

中文翻译：

---

14C-豆荚灌浆过程中不同水分水平下菜豆（Phaseolus vulgaris L.）的分配和生物量分配

植物改变其碳分配以应对低可用水量。本研究的目的是使用¹⁴ CO ₂脉冲追踪分析来评估水分限制对普通豆类植物生物量生产的影响。奥蒂。植物保持灌溉，直到豆荚开始填充；然后，形成三个组，保持在 100%、75% 或 50% 的场容量 (FC)。10天后，向植物供应¹⁴ CO _{2气体4小时。}在贴上标签后 1、3 和 7 天收获植物。成熟水果进口占总量的50%以上¹⁴C. 尤其是豆荚发育第三阶段的果皮变化较大。果糖浓度是葡萄糖的两倍，并随着结荚年龄而降低；与第二阶段相比，豆荚发育阶段 III 和 IV 果皮中的蔗糖浓度增加。蔗糖仅在第 7 天的 II 期果皮中减少，淀粉浓度在 50% FC 条件下减少了一半。后者与天然凝胶中评估的最高淀粉分解活性一致。这些发现为研究水分限制下的碳分配机制开辟了新的机会。