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Dietary administration of tyramine upregulates on immune resistance, carbohydrate metabolism, and biogenic amines in Macrobrachium rosenbergii
Developmental & Comparative Immunology ( IF 2.7 ) Pub Date : 2021-08-21 , DOI: 10.1016/j.dci.2021.104236
Hsin-Wei Kuo, Winton Cheng

Survival rates of prawn, Macrobrachium rosenbergii, against Lactococcus garvieae, immune parameters, carbohydrate metabolism and biogenic amines were determined when the prawn were fed diets containing tyramine (TA) at the levels of 1 and 10 mg kg−1. Results showed that prawn fed diets containing TA for 3 days, challenged with L. garvieae, and then continuously fed individually tested diets had a significantly higher survival rate than those fed the control diet at 168 h after the challenge, in a dose-dependent manner. Results included significant increases in total haemocyte count (THC), granular cells (GCs), semigranular cells (SGCs), phenoloxidase (PO) activity, respiratory bursts (RBs), RBs per haemocyte, phagocytic activity, and clearance efficiency to L. garvieae. Significantly lower plasma glucose and lactate were observed in prawn fed with TA-containing diets for 3 days, a result consistent with increases in the survival rate of the challenge test and the haemolymph octopamine (OA) level. Haemolymph dopamine (DA), norepinephrine (NE), TA, and OA levels of prawn that were fed TA-supplemented diets increased significantly after 1 day, and OA level increased continuously until the third day with a dose-effect relationship. It is therefore concluded that TA can be absorbed from a TA-containing diet to elevate haemolymph TA level, inducing the release of DA, NE, and OA to maintain homeostasis. The higher, more extensive OA expression promoted carbohydrate metabolism and immune resistance in M. rosenbergii.



中文翻译:

酪胺的膳食管理上调罗氏沼虾的免疫抵抗、碳水化合物代谢和生物胺

当对虾饲喂含有 1 和 10 mg kg -1水平的酪胺 (TA) 的饲料时,测定了罗氏沼虾( Macrobrachium rosenbergii ) 对Lactococcus garvieae的存活率、免疫参数、碳水化合物代谢和生物胺。结果表明,对虾饲喂含有 TA 的饲料 3 天,用L. garvieae 攻击,然后在攻击后 168 小时连续饲喂单独测试的饮食具有显着高于对照饮食的存活率,呈剂量依赖性。结果包括总血细胞计数 (THC)、颗粒细胞 (GC)、半颗粒细胞 (SGC)、酚氧化酶 (PO) 活性、呼吸爆发 (RB)、每个血细胞的 RB、吞噬活性和对L. garvieae的清除效率显着增加. 在喂食含 TA 饲料 3 天的对虾中观察到显着降低的血浆葡萄糖和乳酸,这一结果与攻击试验的存活率和血淋巴章鱼胺 (OA) 水平的增加一致。1 天后,添加 TA 饲料的虾的血淋巴多巴胺 (DA)、去甲肾上腺素 (NE)、TA 和 OA 水平显着增加,并且 OA 水平持续增加,直至第三天,呈剂量-效应关系。因此得出结论,可以从含 TA 的饮食中吸收 TA,以提高血淋巴 TA 水平,诱导 DA、NE 和 OA 的释放以维持体内平衡。更高、更广泛的 OA 表达促进了罗氏沼虾的碳水化合物代谢和免疫抵抗。

更新日期:2021-08-29
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