Background

The species Capoeta aculeata (Valenciennes, 1844) is one of the most important freshwater species endemic to Iran. However, the investigation of the population genetic structure of this species is limited by the low number of molecular markers currently described.

Methods and results

In this study, we implemented next generation sequencing technology to identify polymorphic microsatellite markers and investigate the population genetic structure of C. aculeata sampled from three geographical sites in Iran. We characterized and developed 36 novel polymorphic microsatellite markers and these loci were examined in 120 individuals from three populations occurring in the Zagros basin. The average number of alleles per locus varied from 1.7 to 16 (average = 7.89). The results showed that, the polymorphism information content (PIC) of these simple sequence repeat (SSR) loci varied from 0.254 to 0.888. The observed heterozygosity (H_O) per locus ranged from 0.170 to 0.881, while the expected heterozygosity (H_E) per locus was from 0.170 to 0.881. Among these SSR loci, 20 loci deviated significantly from the Hardy–Weinberg equilibrium after Bonferroni correction (p < 0.05).

Conclusions

These microsatellite markers could provide a valuable tool for future population and conservation genetics studies of C. aculeate and other closely related species.

中文翻译：

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基于测序的新型基因分型 (GBS) 的简单序列重复 (SSR) 的表征及其在 Capoeta aculeata 种群基因组学中的应用 (Valenciennes, 1844)

背景

Capoeta aculeata (Valenciennes, 1844) 是伊朗特有的最重要的淡水物种之一。然而，对该物种种群遗传结构的研究受到目前描述的分子标记数量少的限制。

方法和结果

在这项研究中，我们实施了下一代测序技术来识别多态性微卫星标记，并调查从伊朗三个地理地点采样的C. aculeata的种群遗传结构。我们表征并开发了 36 个新的多态微卫星标记，并在来自扎格罗斯盆地三个种群的 120 个个体中检查了这些基因座。每个基因座的平均等位基因数从 1.7 到 16 不等（平均值 = 7.89）。结果表明，这些简单序列重复（SSR）位点的多态性信息含量（PIC）从0.254到0.888不等。每个位点观察到的杂合度 ( H _O ) 范围为 0.170 到 0.881，而预期杂合度 ( H _E) 每个基因座从 0.170 到 0.881。在这些 SSR 位点中，有 20 个位点在 Bonferroni 校正后显着偏离了 Hardy-Weinberg 平衡（p  < 0.05）。

结论

这些微卫星标记可以为C.aculeate和其他密切相关物种的未来种群和保护遗传学研究提供有价值的工具。