Pseudomonas aeruginosa (P. aeruginosa) is a life-threatening pathogen associated with multiantibiotic resistance, which is largely caused by its strong ability to form biofilms. Recent research has revealed that gallium (III) shows an activity against the biofilm of P. aeruginosa by interfering with Fe metabolism. The antibacterial activity of the combination of Ga³⁺ ion and antibiotic rifampicin (RMP) against P. aeruginosa PAO1 is investigated. An anionic polymer poly{{2-[(2-methylprop-2-enoyl)oxy]ethyl}phosphonic acid} (PDMPOH) is exploited to form complexes (GaPD) with Ga³⁺. The GaPD complexes act as a carrier of Ga³⁺ and release Ga³⁺ via enzymatic degradation by bacterial lipases. GaPD is found to damage the outer membrane, leading to enhanced cellular uptake of RMP and Ga³⁺ due to increased outer membrane permeability, which inhibits the RNA polymerase and interferes with Fe metabolism. The antibiofilm activity and biocompatibility of the GaPD system offer a promising treatment option for P. aeruginosa biofilm-related infections.

中文翻译：

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镓 (III) 复合阴离子聚合物与抗生素联合使用的抗生物膜活性

铜绿假单胞菌( P. aeruginosa ) 是一种威胁生命的病原体，与多抗生素耐药性相关，这主要是由于其形成生物膜的能力很强。最近的研究表明，镓 (III)通过干扰铁代谢显示出对抗铜绿假单胞菌生物膜的活性。研究了 Ga ³⁺离子和抗生素利福平 (RMP) 的组合对铜绿假单胞菌PAO1的抗菌活性。利用阴离子聚合物聚{{2-[(2-甲基丙-2-烯酰基)氧基]乙基}膦酸}(PDMPOH)与Ga ³⁺形成络合物(GaPD) 。GaPD 配合物作为 Ga ³⁺的载体并释放 Ga ³⁺通过细菌脂肪酶的酶促降解。研究发现 GaPD 会损伤外膜，导致外膜通透性增加，从而导致细胞对 RMP 和 Ga ^{3+ 的}吸收增强，从而抑制 RNA 聚合酶并干扰 Fe 代谢。GaPD 系统的抗生物膜活性和生物相容性为铜绿假单胞菌生物膜相关感染提供了有希望的治疗选择。