Nepeta binaloudensis Jamzad is an Iranian endemic and rare plant species. This endangered species needs to be urgently protected due to excessive harvesting and habitat destruction. Nepeta species have played a crucial role in Iranian traditional medicine to treat disorders. Plant tissue culture can make a significant contribution to the conservation of endangered plants. In this study, an effort has been made for the efficient regeneration of N. binaloudensis by in vitro tissue culture. For this purpose, the experiments were set up to characterize the potential role of explants (the shoot tip, leaf, or stem), media (MS, ½ MS, and B5), and different concentrations of BAP alone or in combination with IAA for increasing N. binaloudensis propagation. Analysis of some morphological characteristics showed that stem-segment explant and macro-half strength (½ MS) medium were appropriate for inducing shoot formation. Enhancing shoot formation was correlated with an increased concentration of 1.5 mg L⁻¹ BAP in combination with 1.5 mg L⁻¹ IAA. An increase in IAA concentrations led to modulation of adventitious root formation, where the maximum rate of root formation was assayed under a combination of 1.5 mg L⁻¹ IAA and 1.5 mg L⁻¹ BAP. Results showed that about 30% of soil-cultivated plantlets survived when transferred to the field condition. This study describes a rapid protocol to conserve N. binaloudensis to meet future demand.

Nepeta binaloudensis Jamzad 是伊朗特有的稀有植物。由于过度捕捞和栖息地破坏，这种濒危物种需要紧急保护。荆芥属在伊朗治疗疾病的传统医学中发挥了关键作用。植物组织培养可以为濒危植物的保护做出重大贡献。在这项研究中，我们努力通过体外组织培养有效地再生N. binaloudensis。为此，设置实验以表征外植体（茎尖、叶或茎）、培养基（MS、½ MS 和 B5）以及不同浓度的 BAP 单独或与 IAA 组合的潜在作用。增加N。binaloudensis繁殖。对一些形态特征的分析表明，茎段外植体和宏观半强度 (½ MS) 培养基适合诱导芽形成。增强芽形成与1.5mg L ^-1 BAP 与1.5mg L ^-1 IAA组合的浓度增加相关。IAA浓度的增加导致不定根形成的调节，其中根形成的最大速率在1.5mg L ^-1 IAA和1.5mg L ^-1 BAP的组合下测定。结果表明，大约30%的土壤栽培小苗在转移到田间条件下成活。本研究描述了一种保存N的快速协议。比纳卢德亚种 以满足未来的需求。