Anticancer effect of resveratrol and the role of sodium/lithium/calcium exchanger in context with calcium ions are studied in human cervical cancer cell line. This therapeutic approach using siNCLX mediated gene silencing and drug therapy with resveratrol indicates the disruption of calcium homeostasis, increase in caspase (-3, 8, 9) mRNA expressions and DNA damage leading to apoptotic cell death. Monitoring the intracellular Ca²⁺ changes using fluo-4AM indicates highest rise in [Ca²⁺] level in sodium/lithium/calcium exchanger silenced group with five different stages, that is distinguishable based on the fluorescence intensity. In resveratrol treated and siNCLX + resveratrol treated groups no such cell staging differences were observed, despite uniform Ca²⁺ rise followed by decrease in the intensity. Integrating RNAi gene silencing of sodium/lithium/calcium exchanger with resveratrol can form the most interesting, efficient and promising therapeutic strategy in the treatment of cancer.

在人宫颈癌细胞系中研究了白藜芦醇的抗癌作用以及钠/锂/钙交换剂与钙离子的作用。这种使用 siNCLX 介导的基因沉默和白藜芦醇药物治疗的治疗方法表明，钙稳态被破坏，caspase (-3,8,9) mRNA 表达增加，DNA 损伤导致细胞凋亡。使用fluo-4AM 监测细胞内Ca ²⁺变化表明，钠/锂/钙交换器沉默组中[Ca ²⁺ ] 水平升高最高，具有五个不同阶段，可根据荧光强度进行区分。在白藜芦醇处理组和siNCLX + 白藜芦醇处理组中，尽管Ca ²⁺均匀上升，随后强度下降，但没有观察到这种细胞分期差异。将钠/锂/钙交换体的RNAi基因沉默与白藜芦醇整合可以形成癌症治疗中最有趣、最有效和最有前途的治疗策略。