Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2021-08-21 , DOI: 10.1007/s00216-021-03619-7 Riikka Peltomaa 1, 2 , Rodrigo Barderas 3 , Elena Benito-Peña 4 , María C Moreno-Bondi 4
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Antibodies are widely employed as biorecognition elements for the detection of a plethora of compounds including food and environmental contaminants, biomarkers, or illicit drugs. They are also applied in therapeutics for the treatment of several disorders. Recent recommendations from the EU on animal protection and the replacement of animal-derived antibodies by non-animal-derived ones have raised a great controversy in the scientific community. The application of recombinant antibodies is expected to achieve a high growth rate in the years to come thanks to their versatility and beneficial characteristics in comparison to monoclonal and polyclonal antibodies, such as stability in harsh conditions, small size, relatively low production costs, and batch-to-batch reproducibility. This review describes the characteristics, advantages, and disadvantages of recombinant antibodies including antigen-binding fragments (Fab), single-chain fragment variable (scFv), and single-domain antibodies (VHH) and their application in food analysis with especial emphasis on the analysis of biotoxins, antibiotics, pesticides, and foodborne pathogens. Although the wide application of recombinant antibodies has been hampered by a number of challenges, this review demonstrates their potential for the sensitive, selective, and rapid detection of food contaminants.
Graphical abstract
中文翻译：
重组抗体及其在食品免疫分析中的应用
抗体被广泛用作生物识别元件，用于检测多种化合物，包括食品和环境污染物、生物标志物或非法药物。它们还应用于治疗多种疾病。欧盟最近关于动物保护和用非动物源性抗体替代动物源性抗体的建议在科学界引起了很大争议。重组抗体的应用预计将在未来几年实现高增长率，这得益于其与单克隆和多克隆抗体相比的多功能性和有益特性，例如在恶劣条件下的稳定性、小尺寸、相对较低的生产成本和批量-批次重现性。本综述描述了重组抗体（包括抗原结合片段（Fab）、单链片段可变区（scFv）和单域抗体（V HH ））的特点、优点和缺点及其在食品分析中的应用，特别强调生物毒素、抗生素、农药和食源性病原体的分析。尽管重组抗体的广泛应用受到许多挑战的阻碍，但本次综述证明了它们在灵敏、选择性和快速检测食品污染物方面的潜力。
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