Electrospun polymers are an example of multi-functional biomaterials that improve the material-cellular interaction and aimed at enhancing wound healing. The main objective of this work is to fabricate electrospun polyurethane membranes using arginine as chain extender (PUUR) in order to test the fibroblasts affinity and adhesion on the material and the polymer toxicity. Polyurethane membranes were prepared in two steps: (i) the polyurethane synthesis, and ii) the electrospinning process. The membranes were characterized by scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy, gel permeation chromatography, and differential scanning calorimetry techniques. The evaluation of PUUR as a scaffolding biomaterial for growing and developing of cells on the material was realized by LIVE/DEAD staining. The results show that the fluorescent surface area of human fibroblasts (hFB), was greater in control dense membranes made from Tecoflex than in electrospun and dense PUUR. From SEM analysis, the electrospun membranes show relatively uniform attachment of cells with a well-spread shape, while Tecoflex dense membranes show a non-proliferating round shape, which is attributed to the fiber’s structure in electrospun membranes. The cell morphology and the cell attachment assay results reveal the well spreading of hFB cells on the surface of electrospun PUUR membranes which indicates a good response related to cell adhesion.

静电纺丝聚合物是多功能生物材料的一个例子，可改善材料与细胞的相互作用，旨在促进伤口愈合。这项工作的主要目的是使用精氨酸作为扩链剂 (PUUR) 制造电纺聚氨酯膜，以测试成纤维细胞对材料的亲和力和粘附性以及聚合物的毒性。聚氨酯膜的制备分两个步骤：(i) 聚氨酯合成，和 ii) 静电纺丝过程。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱、凝胶渗透色谱和差示扫描量热技术对膜进行了表征。通过 LIVE/DEAD 染色实现了对 PUUR 作为用于在材料上生长和发育细胞的支架生物材料的评估。结果表明，在由 Tecoflex 制成的对照致密膜中，人成纤维细胞 (hFB) 的荧光表面积大于电纺和致密 PUUR 中的荧光表面积。从 SEM 分析来看，电纺膜显示出相对均匀的细胞附着，形状分布良好，而 Tecoflex 致密膜显示出不增殖的圆形，这归因于电纺膜中的纤维结构。细胞形态和细胞附着测定结果显示 hFB 细胞在电纺 PUUR 膜表面上的良好扩散，这表明与细胞粘附相关的良好反应。电纺膜显示出相对均匀的细胞附着，形状分布良好，而 Tecoflex 致密膜呈非增殖圆形，这归因于电纺膜中的纤维结构。细胞形态和细胞附着测定结果显示 hFB 细胞在电纺 PUUR 膜表面上的良好扩散，这表明与细胞粘附相关的良好反应。电纺膜显示出相对均匀的细胞附着，形状分布良好，而 Tecoflex 致密膜呈非增殖圆形，这归因于电纺膜中的纤维结构。细胞形态和细胞附着测定结果显示 hFB 细胞在电纺 PUUR 膜表面上的良好扩散，这表明与细胞粘附相关的良好反应。