The zinc oxide nanoparticles (T-ZnO NPs) were synthesized successfully by the application of leaf, stem and callus extracts of Tabernaemontana heyneana Wall. via the green combustion method. The structural, bonding and morphological features of T. heyneana mediated ZnO NPs (T-ZnO NPs) were explored using XRD (X-ray powder diffraction), UV–Vis (Ultra-violet visible spectroscopy), FESEM-EDS (Field Emission-SEM and energy-dispersive X-ray spectra), DLS (Dynamic light scattering) and FTIR (Fourier transform infrared) techniques. UV–Vis spectra revealed the presence of a band in the region between 370 and 376 nm approving the presence of T-ZnO NPs. XRD, FESEM-EDS, TEM and DLS analysis confirmed the formation of nanosized, spherical shaped, highly stable and pure crystalline wurtzite T-ZnO NPs. FTIR spectra revealed the presence of most probable phytochemicals from plant extracts involved in the processes of reduction and stabilization of T-ZnO NPs. The T-ZnO NPs could effectively inhibit the activity of DPPH (1, 1-Diphenyl-2-picrylhydrazyl) radical (IC₅₀ value between 467.7 and 752.3 µg/ml) exhibiting potent radical scavenging activity. The T-ZnO NPs exhibited strong anti-inflammatory (membrane stabilization) activity. The antidiabetic potential of T-ZnO NPs was assessed and found to exhibit excellent α-glucosidase (IC ₅₀ at 16.3 µg/ml) and α-amylase (IC ₅₀ at 42.3 µg/ml) activity. The cytotoxic effect of T-ZnO NPs was investigated against A549 cell line (human lung cancer) by MTT assay which showed dose dependent response. IC₅₀ values were found to be in the range of 89.47–185.8 µg/ml demonstrating their higher cytotoxic efficacy. AO/EB staining indicated the presence of early apoptotic cells. The percentage degradation of carcinogenic methylene blue dye was studied and an outstanding catalytic activity of 87–100% was recorded by ZnO-NPs within 160 min. Hence, T-ZnO NPs proved to be better candidates for futuristic biomedical and industrial applications.

应用Tabernaemontana heyneana Wall的叶、茎和愈伤组织提取物成功合成了氧化锌纳米颗粒（T- ZnO NPs）。通过绿色燃烧法。的结构，粘结和形态特征T. heyneana介导的氧化锌纳米颗粒（Ť -ZnO纳米颗粒）用XRD（X射线粉末衍射），UV-VIS（紫外可见分光法），FESEM-EDS（现场Emission-探索SEM 和能量色散 X 射线光谱）、DLS（动态光散射）和 FTIR（傅里叶变换红外）技术。UV-Vis 光谱显示在 370 和 376 nm 之间的区域存在一条带，证明T的存在-ZnO 纳米颗粒。XRD、FESEM-EDS、TEM 和 DLS 分析证实了纳米尺寸、球形、高度稳定和纯结晶纤锌矿T- ZnO NPs 的形成。FTIR 光谱揭示了植物提取物中最可能存在的植物化学物质，这些植物化学物质与T- ZnO NPs的还原和稳定过程有关。所述Ť -ZnO纳米颗粒能有效地抑制（IC DPPH的活性（1，1-二苯基-2-苦基肼）基团₅₀表现出强效的自由基清除活性和467.7 752.3微克/毫升之间的值）。所述Ť -ZnO纳米颗粒表现出较强的抗炎（膜稳定）的活性。T的抗糖尿病潜力对 -ZnO NPs 进行了评估，发现其表现出优异的 α-葡萄糖苷酶（IC ₅₀为 16.3 µg/ml）和 α-淀粉酶（IC ₅₀为 42.3 µg/ml）活性。通过 MTT 测定研究了T- ZnO NPs 对 A549 细胞系（人肺癌）的细胞毒性作用，显示出剂量依赖性反应。发现IC ₅₀值在 89.47–185.8 µg/ml 的范围内，表明它们具有更高的细胞毒性功效。AO/EB 染色表明存在早期凋亡细胞。研究了致癌亚甲蓝染料的降解百分比，ZnO-NPs 在 160 分钟内记录了 87-100% 的出色催化活性。因此，T-ZnO NPs 被证明是未来生物医学和工业应用的更好候选者。