A new and sensitive (TLC) assay has been estimated for quantitation of synthetic binary mixtures of antipsychotic drug Amisulpride (AMS) with antimigarine drug Zolmitriptan (ZOL). These medications co-administered with each other for treatment of chronic migraine headache associated with psychosis. The synthetic mixture has been separated on precoated silica TLC plates G60 F254 by using mobile phase which consist of mixture of Chloroform: Ethyl acetate: Methanol: Ammonia in ratio (30:30:73:3 v/v/v/v). The mixture has been determined at λ_max 231 nm. Retardation factor (RF) values for AMS and ZOL have been 0.6 and 0.43 respectively. Calibration graphs have been linear in range 50–500 ng/spot for all studied drugs. The detection limits have been 6.04 & 10.5 ng/spot and the quantitation limits have been 18.3 & 31.834 ng/spot for AMS and ZOL respectively. The described assay has been utilized for quantitation of synthetic mixture in pharmaceutical tablet &biological fluids.

一种新的灵敏 (TLC) 测定已被估计用于定量抗精神病药物氨磺必利 (AMS) 与抗偏头痛药物佐米曲坦 (ZOL) 的合成二元混合物。这些药物相互联合给药，用于治疗与精神病相关的慢性偏头痛。使用由氯仿：乙酸乙酯：甲醇：氨的比例（30:30:73:3 v/v/v/v）组成的流动相在预涂硅胶 TLC 板 G60 F254 上分离合成混合物。混合物已在 λ _max处确定231 纳米。AMS 和 ZOL 的延迟因子 (RF) 值分别为 0.6 和 0.43。所有研究药物的校准图在 50–500 ng/点范围内呈线性。AMS 和 ZOL 的检测限分别为 6.04 和 10.5 ng/点，定量限分别为 18.3 和 31.834 ng/点。所描述的测定已用于定量药片和生物流体中的合成混合物。