Dengue virus and Chikungunya virus are arboviruses that affect thousands of people worldwide annually. The mechanisms involved in viral pathogenesis still need to be better understood. Single nucleotide polymorphisms (SNPs) in immune genes may be involved in the protection, susceptibility, and/or progression of these diseases. This study was performed to investigate the SNP −174 G/C in the interleukin-6 (IL-6) gene in patients with dengue or chikungunya from Northeastern Brazil. A total of 581 blood samples were analyzed, of which 244 were part of the negative control group, genomic DNA was extracted, and the SNP was genotyped using real-time polymerase chain reaction (PCR). The data obtained were used to conduct statistical analyses of the genotype and allele frequencies. We suggest that the G/C genotype and C allele of the SNP −174 G/C in the IL-6 gene are related to protection against dengue in the studied population. No significant differences were observed in chikungunya patients. This is the first study that assessed the association of the SNP −174 G/C in patients with chikungunya. We identified the presence of the C allele as a protective factor against dengue in the studied population.

中文翻译：

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虫媒病毒感染中白细胞介素 6 基因 -174 G/C 多态性的患病率和影响

登革热病毒和基孔肯雅病毒是每年影响全世界数千人的虫媒病毒。仍然需要更好地了解病毒发病机制中涉及的机制。免疫基因中的单核苷酸多态性 (SNP) 可能与这些疾病的保护、易感性和/或进展有关。本研究旨在调查白细胞介素 6 ( IL-6) 来自巴西东北部的登革热或基孔肯雅热患者的基因。共分析了581份血液样本，其中244份为阴性对照组，提取基因组DNA，使用实时聚合酶链反应（PCR）对SNP进行基因分型。获得的数据用于对基因型和等位基因频率进行统计分析。我们认为IL-6基因中 SNP -174 G/C 的 G/C 基因型和 C 等位基因与研究人群中的登革热保护有关。在基孔肯雅病患者中未观察到显着差异。这是第一项评估基孔肯雅病患者 SNP -174 G/C 相关性的研究。我们将 C 等位基因的存在确定为研究人群中针对登革热的保护因素。